Silibinin, up-regulates chemokine receptor expression in MDA-MB-231 breast cancer cell line

Authors

  • Nikoo Khannazer Department of Molecular and Cellular Biology, College of Science, University ofTehran, Tehran
  • Seyed Hassan Paylakhi School of Biology, Damghan University, Damghan
  • Abbas Mirshafiey Departmant of Immunology, School of Public Health, Tehran University of MedicalSciences, Tehran
  • Gholamreza Azizi Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj
  • Nasrin Motamed Department of Molecular and Cellular Biology, College of Science, University of Tehran

DOI:

https://doi.org/10.3329/bjms.v14i2.16740

Keywords:

Silibinin, MDA-MB-231, CXCR3, CCR5, CCR7

Abstract

Introduction: Silibinin, a polyphenolic flavonoid isolated from the milk thistle plant (Silybummarianum), has various applications in cancer therapy. This investigation aimed to examine the effects of silibinin on proliferation and chemokine receptor expression on MDA-MB-231 cells, a highly metastatic human breast cancer cell line.

Methods: The cytotoxic effect of silibinin on MDA-MB-231 cells was determined by micro-culture tetrazolium test (MTT) assay. In addition, the expression of chemokine receptors CXCR3, CCR5 and CCR7 genes in response to silibinin was evaluated by Real-Time PCR.

Results: Data analysis from MTT assay showed that silibinin had dose-dependent and time-dependent inhibitory effects on MDA-MB-231 cell line. Moreover, Real-Time PCR analysis showed that silibinin not only had no inhibitory effects on CXCR3, CCR5 and CCR7 gene expressions, but also could increase significantly the expression of these genes in a dose and time-dependent manner.

Conclusion: These results revealed for the first time the increased possibility of CXCR3, CCR5 and CCR7 genes expression in response to silibinin in human breast cancer cells.

Bangladesh Journal of Medical Science Vol.14(2) 2015 p.190-195

Downloads

Download data is not yet available.
Abstract
253
PDF
170

Downloads

Published

2015-04-18

How to Cite

Khannazer, N., Paylakhi, S. H., Mirshafiey, A., Azizi, G., & Motamed, N. (2015). Silibinin, up-regulates chemokine receptor expression in MDA-MB-231 breast cancer cell line. Bangladesh Journal of Medical Science, 14(2), 190–195. https://doi.org/10.3329/bjms.v14i2.16740

Issue

Vol. 14 No. 2 (2015)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.