Eriocalyxin B inhibits proliferation and induces apoptosis through down-regulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells

Authors

  • Azhar Rasul The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Jinmei Liu The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Ying Liu Dental Hospital, Jilin University, Changchun 130041
  • Faya Martin Millimouno The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Xiaowan Wang The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Ichiro Tsuji Department of Public Health, Tohoku University, Sendai 9808576
  • Takaki Yamamura Food and Nutrition, Morioka College, Iwate
  • Jiang Li Dental Hospital, Jilin University, Changchun 130041
  • Xiaomeng Li The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024

DOI:

https://doi.org/10.3329/bjp.v8i2.13797

Keywords:

Eriocalyxin B, Bladder cancer, T24 cells, Apoptosis

Abstract

Eriocalyxin B (EriB) has been shown to possess promising anticancer potential against various cancer cells. However, its effect against bladder cancer cells remained unexplored. In this study, for the first time, we investigated the effects of EriB on cell proliferation, cell cycle, and apoptosis in bladder cancer T24 cells. In order to examine the effects of EriB on cell proliferation, cell cycle, and apoptosis, we performed MTT assay, flow cytometric analysis and western blot. The results revealed that EriB decreased the cell viability of T24 cells. Flow cytometric analysis demonstrated that EriB markedly induced apoptosis of T24 cells and arrested cell cycle at G2/M phase in a dose-dependent manner. Further characterization showed that EriB involved in the down-regulation of Bcl-2 and activation of caspase-3 before culminating in apoptosis in EriB-treated T24 cells. These in vitro results suggested that EriB should be further examined for in vivo activity in human bladder cancer.

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Author Biography

Azhar Rasul, The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024

Postdoctoral Research Fellow

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Published

2013-03-08

How to Cite

Rasul, A., J. Liu, Y. Liu, F. M. Millimouno, X. Wang, I. Tsuji, T. Yamamura, J. Li, and X. Li. “Eriocalyxin B Inhibits Proliferation and Induces Apoptosis through down-Regulation of Bcl-2 and Activation of Caspase-3 in Human Bladder Cancer Cells”. Bangladesh Journal of Pharmacology, vol. 8, no. 2, Mar. 2013, pp. 116-23, doi:10.3329/bjp.v8i2.13797.

Issue

Vol. 8 No. 2 (2013)

Section

Research Articles

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