In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

Abstract

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate.

Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration

DOI: 10.3329/bjsir.v44i4.4597

Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009