Bovine herpes virus-1 (BoHV-1) detection in dairy cattle with reproductive problems in Sudan

Authors

  • Amira Mohamed Elhassan Virology Department, Veterinary Research Institute, Khartoum
  • Mohamed Abdalla Fadol Viral Vaccine Production, Veterinary Research Institute, Khartoum
  • Mohamed Ahmed Mohamed Salih Molecular Biology Department, Central Laboratory, Khartoum
  • Abdel Rahim Mohamed El Hussein Virology Department, Veterinary Research Institute, Khartoum

Keywords:

BoHV-1, Dairy cattle, Reproductive problems, Sudan

Abstract

The present work aimed to observe the infection pattern of Bovine herpes virus-1 (BoHV-1) in dairy cattle with reproductive problems in Sudan. A total of 140 samples comprising of vaginal swab (n=97), placenta (n=15), whole blood (n=19), uterine fluid (n=1), and serum (n=8) were collected from 16 dairy herds showing particularly high rate of abortion and infertility in Khartoum State. The samples were used for virus isolation, and were tested by Enzyme-Linked Immunosorbent Assay (ELISA) and polymerase chain reaction (PCR). No virus could be isolated from the samples inoculated for isolation in cell culture. Out of 80 specimens tested by ELISA, 7 (8.75%) were found to be positive, and one sample was doubtful. Using PCR, 11 (10.7%) out of 103 samples were found to be positive. When comparing between two methods for DNA extraction, the DNA extracted by commercial kit was found to be better in quality as compared to the DNA extracted using phenol/chloroform/isoamyl-alcohol method. The study confirmed the presence of BoHV-1 in cattle farms with reproductive problems in Sudan.

 

http://dx.doi.org/10.5455/javar.2015.b81


Downloads

Download data is not yet available.
Abstract
848
PDF
545

Author Biography

Amira Mohamed Elhassan, Virology Department, Veterinary Research Institute, Khartoum

Assistant Professor, Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202, Bangladesh

Downloads

Published

2015-05-20

How to Cite

Elhassan, A. M., Fadol, M. A., Salih, M. A. M., & El Hussein, A. R. M. (2015). Bovine herpes virus-1 (BoHV-1) detection in dairy cattle with reproductive problems in Sudan. Journal of Advanced Veterinary and Animal Research, 2(2), 185–189. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/23217

Issue

Vol. 2 No. 2 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).