<i>In vitro</i> propagation of <i>Citrullus lanatus</i> Thumb. from nodal explants culture

Authors

  • MM Khatun Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi
  • MS Hossain Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi
  • MA Haque Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh
  • M Khalekuzzaman Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi

Keywords:

In vitro regeneration, Nodal explant, Citrullus lanatus

Abstract

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully.

Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus

DOI: 10.3329/jbau.v8i2.7926

J. Bangladesh Agril. Univ. 8(2): 203-206, 2010  

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How to Cite

Khatun, M., Hossain, M., Haque, M., & Khalekuzzaman, M. (2011). <i>In vitro</i> propagation of <i>Citrullus lanatus</i> Thumb. from nodal explants culture. Journal of the Bangladesh Agricultural University, 8(2), 203–206. Retrieved from https://www.banglajol.info/index.php/JBAU/article/view/7926

Issue

Vol. 8 No. 2 (2010)

Section

Crop Science

License

© 2003-2017 Bangladesh Agricultural University Research System.

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