Plant Regeneration from Seedling Derived Explants through Callus of Eggplant (<i>Solanum melongena</i> L)

Abstract

Different concentrations and combinations of hormones were used in MS medium to observe callus induction and then plant regeneration using stem, leaf and root explants. The rate of callus formation varied in different treatments used. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem after 8.2 days. The highest fresh weight of callus was 1.12, 0.5 and 0.48 g from stem, leaf and root, respectively. The highest percentage of regeneration (23.28%) was recorded in MS media containing 2.0 mg/l BAP + 0.5 mg/l NAA from stem after 38.8 days. However, 11.94% leaf the regeneration was obtained by 2.0 mg/l BAP + 0.5 mg/l NAA after 46.2 days. It was also found that 2.0 mg/l BAP + 0.5 mg/l NAA was the best stem regeneration. The protocol in the study might be useful for the production of disease free and healthy plant materials and also it would be useful for genetic transformation of eggplant using biotechnological approach.

Keywords: 6-Benzyl amino purine (BAP); α-Naphthalene acetic acid (NAA); Solanum melongena; regeneration.

DOI: 10.3329/agric.v8i2.7583

The Agriculturists 8(2): 98-107 (2010)