Dietary supplementation of fenugreek (Trigonella foenum-graecum) seed as an alternative to antibiotics on growth performance and health of broiler chickens
DOI:
https://doi.org/10.3329/ajmbr.v11i4.83242Keywords:
phytogenic additives, hematological parameters, immune response, gut microbiota, natural growth promoterAbstract
Growing concerns over the use of antibiotics in poultry production have led to increased exploration of natural feed additives with health-promoting benefits. This study aimed to assess the impact of fenugreek seed (Trigonella foenum-graecum) supplementation in broiler diets as a potential substitute for antibiotic growth promoters. Conducted over a 28-day period (May 8 to June 5, 2019) at the Sher-e-Bangla Agricultural University Poultry Farm in Dhaka, the trial involved 150 Cobb 500 day-old broiler chicks. The birds were distributed randomly across five dietary groups, each with three replicates containing 10 chicks. The treatment groups included a control (no additive), an antibiotic-supplemented diet, and diets supplemented with fenugreek seed at 1%, 1.5%, and 2% inclusion levels. The findings revealed a significant (P<0.05) enhancement in final body weight and dressing percentage in birds fed fenugreek, with the 1.5% group achieving the highest body weight (1528.33 ± 57.47 g). Although feed conversion ratio (FCR) did not show significant variation across treatments, birds receiving the 1% fenugreek diet consumed the most feed (2289.67 ± 2.60 g). No significant differences (P>0.05) were observed in the relative weights of internal organs or immune tissues. Blood cholesterol levels significantly decreased in the fenugreek-fed groups, while glucose levels remained unchanged. Hematological profiles improved notably in treated birds, particularly in hemoglobin concentration, red and white blood cell counts, and lymphocyte percentages. Microbial analysis showed that fenugreek supplementation reduced pathogenic bacteria such as E. coli and Salmonella, while promoting beneficial Lactobacillus populations. Additionally, antibody titers against Newcastle disease were significantly higher (P<0.05) in fenugreek-treated groups. Incorporating fenugreek seed into broiler diets positively influenced growth metrics, enhanced immune function, improved gut microbial balance, and supported key hematological parameters. These outcomes highlight fenugreek seed as a promising natural alternative to synthetic antibiotics in broiler production systems.
Asian J. Med. Biol. Res. 2025, 11(4), 73-87
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Abd El-Hack ME, MT El-Saadony, HM Salem, AM El-Tahan, MM Soliman, GBA Youssef, AE Taha, SM Soliman, AE Ahmed, AF El-kott, KMA Syaad and AA Swelum, 2022. Alternatives to antibiotics for organic poultry production: types, modes of action and impacts on bird’s health and production. Poult. Sci., 101: 101696.
Ahmed SK, 2014. The off feeding intervals effect on some performance traits of broilers. Iraqi J. Vet. Med., 38: 48-55.
Ali AH, T Yeasmin, YA Mohamed, Aİ Mohamud and P Mishra, 2021. Evaluation of dietary supplementation of fenugreek seed (Trigonella foenum-graecum L.) as a growth promoter in broiler diet and its impacts on growth performance, carcass quality and cost effectiveness. J. Istanbul Vet. Sci., 5: 6-12.
Arif M, M Akteruzzaman, TA Ferdous, SS Islam, BC Das, MP Siddique and SML Kabir, 2021. Dietary supplementation of Bacillus-based probiotics on the growth performance, gut morphology, intestinal microbiota and immune response in low biosecurity broiler chickens. Vet. Anim. Sci., 14: 100216.
Awad E, R Cerezuela and MÁ Esteban, 2015. Effects of fenugreek (Trigonella foenum graecum) on gilthead seabream (Sparus aurata L.) immune status and growth performance. Fish Shellfish Immunol., 45: 454-464.
Camlica M and G Yaldiz, 2024. Comparison of twenty selected fenugreek genotypes grown under irrigated and dryland conditions: morphology, yield, quality properties and antioxidant activities. Agronomy, 14: 713.
Castro FLS, L Chai, J Arango, CM Owens, PA Smith, S Reichelt, C DuBois and A Menconi, 2023. Poultry industry paradigms: connecting the dots. J. Appl. Poult. Res., 32: 100310.
Ceylan N, E Yenice, İ Yavaş, AA Çenesiz, NN Toprak and İ Çiftçi, 2023. Comparative effects of medium‐chain fatty acids or phytobiotics‐based feed additives on performance, caecum microbiota, volatile fatty acid production and intestinal morphology of broilers. Vet. Med. Sci., 9: 2719-2730.
Clark CEF, Y Akter, A Hungerford, P Thomson, MR Islam, PJ Groves and CJ O’Shea, 2019. The intake pattern and feed preference of layer hens selected for high or low feed conversion ratio. PLoS One, 14: e0222304.
Elamin MH, TE Abbas, TA Algam and EM Ahmed, 2020. Effect of supplementation of fenugreek seeds to the broiler chicks diet on the growth performance and carcass characteristics. Pakistan J. Nutr., 19:485-490.
Essien C and G Josiah, 2024. Effect of fenugreek seed powder as feed additive on growth performance, carcass, organ characteristics and nutrient digestibility of finisher broiler chickens. AKSU J. Agric. Food Sci., 8: 121-131.
Faisal Z, R Irfan, N Akram, HMI Manzoor, MA Aabdi, MJ Anwar, S Khawar, A Saif, YA Shah, M Afzaal and DT Desta, 2024. The multifaceted potential of fenugreek seeds: from health benefits to food and nanotechnology applications. Food Sci. Nutr., 12: 2294-2310.
Fawaz MA, AAAM El-Hafez, YA Abdelmoati, AHH Ali and HA Hassan, 2025. Physiological, nutritional, and productive responses of broilers supplemented with lemon, fenugreek, and sesame oils as feed additives. Sci. Rep., 15: 24993.
Giubilini A, P Birkl, T Douglas, J Savulescu and H Maslen, 2017. Taxing meat: taking responsibility for one’s contribution to antibiotic resistance. J. Agric. Environ. Ethics, 30: 179-198.
Hamid MA, MA Rahman, S Ahmed and KM Hossain, 2016. Status of poultry industry in Bangladesh and the role of private sector for its development. Asian J. Poult. Sci., 11: 1-13.
Handley JA, SH Park, SA Kim and SC Ricke, 2018. Microbiome profiles of commercial broilers through evisceration and immersion chilling during poultry slaughter and the identification of potential indicator microorganisms. Front. Microbiol., 9: 345.
Heshmat‐Ghahdarijani K, N Mashayekhiasl, A Amerizadeh, ZT Jervekani and M Sadeghi, 2020. Effect of fenugreek consumption on serum lipid profile: a systematic review and meta‐analysis. Phyther. Res., 34: 2230-2245.
Hina S, S Mustafa, AM Ismail, S Mahmood, A Matar, AS Alharbi, HAF Saleh and W Ge, 2025. Therapeutic and protective valuation of fenugreek (Trigonella foenum-graecum). Ital. J. Food Sci., 37: 1-17.
Huang H, X Wang, L Yang, W He, T Meng, K Zheng, X Xia, Y Zhou, J He, C Liu, S Zou and D Xiao, 2022. The effects of fenugreek extract on growth performance, serum biochemical indexes, immunity and NF-κB signaling pathway in broiler. Front. Vet. Sci., 9: 882754.
Kerek Á, Á Szabó, PF Dobra, K Bárdos, B Paszerbovics, Z Bata, V Molnár-Nagy, Á Jerzsele and L Ózsvári, 2025. Dose–response study of a fenugreek-based antibiotic alternative in Bábolna Tetra-SL chicks (1–42 days old) with mixed bacterial infections. Front. Vet. Sci., 12: 1570387.
Ma F, S Xu, Z Tang, Z Li and L Zhang, 2021. Use of antimicrobials in food animals and impact of transmission of antimicrobial resistance on humans. Biosaf. Heal., 3: 32-38.
Miyakawa MEF, NA Casanova and MH Kogut, 2024. How did antibiotic growth promoters increase growth and feed efficiency in poultry? Poult. Sci., 103: 103278.
Mohammed NI, ZA Abdulkareem, A Abdollahi, HA Khdir, OR Ghaffar, OR Ahmed, RH Arif, HA Mahmood, SI Mustafa and HB Khdhir, 2023. Effects of black cumin, fenugreek, and sesame seeds as a mixture on performance, intestinal morphology, and blood traits of broilers under chronic heat stress conditions. Ital. J. Anim. Sci., 22: 1134-1150.
Mottet A and G Tempio, 2017. Global poultry production: current state and future outlook and challenges. Worlds Poult. Sci. J., 73: 245-256.
Moustafa EM, MAO Dawood, DH Assar, AA Omar, ZI Elbialy, FA Farrag, M Shukry and MM Zayed, 2020. Modulatory effects of fenugreek seeds powder on the histopathology, oxidative status, and immune related gene expression in Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila. Aquaculture, 515: 734589.
Mow NA, MAH Beg, KBMS Islam, S Ahmed, P Bose and MZ Rahman, 2024. Dried Chlorella powder supplementation: impact on broiler chicken growth, health, and intestinal microflora. J. Biosci. Environ. Res., 1: 4-11.
Obianwuna UE, X Chang, VU Oleforuh-Okoleh, PN Onu, H Zhang, K Qiu and S Wu, 2024. Phytobiotics in poultry: revolutionizing broiler chicken nutrition with plant-derived gut health enhancers. J. Anim. Sci. Biotechnol., 15: 169.
Olayeni TB, EO Okanlawon and TP Akilapa, 2024. Effect of dietary fenugreek powder on growth performance, carcass characteristics and serum biochemistry of broiler chicken at starter and finisher phase. Cogniz. J. Multidiscip. Stud., 4: 255-266.
Paneru D, G Tellez-Isaias, MA Arreguin-Nava, N Romano, WG Bottje, E Asiamah, AAA Abdel-Wareth and J Lohakare, 2023. Effect of fenugreek seeds and Bacillus-based direct-fed microbials on the growth performance, blood biochemicals, and intestinal histomorphology of broiler chickens. Front. Vet. Sci., 10: 1298587.
Paneru D, G Tellez-Isaias, WG Bottje, E Asiamah, AAA Abdel-Wareth, M Salahuddin and J Lohakare, 2024. Immune modulation and cecal microbiome changes in broilers fed with fenugreek seeds and Bacillus-based probiotics. Poult. Sci., 103: 104130.
Paneru D, G Tellez-Isaias, N Romano, G Lohakare, WG Bottje and J Lohakare, 2022. Effect of graded levels of fenugreek (Trigonella foenum-graecum L.) seeds on the growth performance, hematological parameters, and intestinal histomorphology of broiler chickens. Vet. Sci., 9: 207.
Peng C, M Ghanbari, A May and T Abeel, 2024. Effects of antibiotic growth promoter and its natural alternative on poultry cecum ecosystem: an integrated analysis of gut microbiota and host expression. Front. Microbiol., 15: 1492270.
Qureshi S, MT Banday, I Shakeel, S Adil, MS Mir, YA Beigh and U Amin, 2016. Histomorphological studies of broiler chicken fed diets supplemented with either raw or enzyme treated dandelion leaves and fenugreek seeds. Vet. World, 9: 269-275.
Rafiq K, MT Hossain, R Ahmed, MM Hasan, R Islam, MI Hossen, SN Shaha and MR Islam, 2022. Role of different growth enhancers as alternative to in-feed antibiotics in poultry industry. Front. Vet. Sci., 8: 794588.
Rahimian Y, SM Akbari, M Karami and M Fafghani, 2018. Effect of different levels of fenugreek powder supplementation on performance, Influenza, sheep red blood cell, New Castle diseases anti-body titer and intestinal microbial flora on Cobb 500 broiler chicks. Banat. J. Biotechnol., IX: 29-37.
Samani SK, MR Ghorbani, J Fayazi and S Salari, 2020. The effect of different levels of Fenugreek (Trigonella foenum-graecum L.) powder and extract on performance, egg quality, blood parameters and immune responses of laying hens in second production cycle. Vet. Res. Int. Q. J., 11: 53-57.
Silva RAD, NE Arenas, VL Luiza, JAZ Bermudez and SE Clarke, 2023. Regulations on the use of antibiotics in livestock production in South America: a comparative literature analysis. Antibiotics, 12: 1303.
Singh N, SS Yadav, S Kumar and B Narashiman, 2022. Ethnopharmacological, phytochemical and clinical studies on Fenugreek (Trigonella foenum-graecum L.). Food Biosci., 46: 101546.
Tiruneh BB, YM Chekol and BA Limenih, 2025. Effect of substituting soybean meal with sweet lupine on the performance of Sasso T44 dual purpose chicken. Sci. Rep., 15: 13997.
Valdez G, LF Shyur, SY Wang and SE Chen, 2023. Phytogenics in ginger, Origanum vulgare, and Syzygium aromaticum and their potential as a feed additive against Clostridium perfringens in broiler production. Animals, 13: 3643.
Valenzuela-Grijalva NV, A Pinelli-Saavedra, A Muhlia-Almazan, D Domínguez-Díaz and H González-Ríos, 2017. Dietary inclusion effects of phytochemicals as growth promoters in animal production. J. Anim. Sci. Technol., 59: 8.
Wang J, L Deng, M Chen, Y Che, L Li, L Zhu, G Chen and T Feng, 2024. Phytogenic feed additives as natural antibiotic alternatives in animal health and production: a review of the literature of the last decade. Anim. Nutr., 17: 244-264.
Weerasingha AS and NSBM Atapattu, 2015. Effects of fenugreek (Trigonella foenum-graecum L.) seed powder on growth performance, visceral organ weight, serum cholesterol levels and the nitrogen retention of broiler or chicken. Trop. Agric. Res., 24: 289.
Yang L, L Chen, K Zheng, YJ Ma, RX He, MA Arowolo, YJ Zhou, DF Xiao and JH He, 2022. Effects of fenugreek seed extracts on growth performance and intestinal health of broilers. Poult. Sci., 101: 101939.
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Copyright (c) 2025 Sadik Ahmed, Noushin Angum Mow, Md Anwarul Haque Beg, Md Mehedi Hasan, Mst Nasrin Banu, Sajibul Hasan, Shanaz Parvin, Md Zaminur Rahman
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