TY - JOUR AU - Ahmed, Sheikh Rashel AU - Howlader, Md Moniruzzaman Shohag AU - Sutradhar, Pijush AU - Yasmin, Sabina PY - 2016/05/15 Y2 - 2024/03/29 TI - An efficient protocol for in vitro regeneration of Stevia rebaudiana JF - Asian Journal of Medical and Biological Research JA - Asian J. Med. Biol. Res. VL - 2 IS - 1 SE - Articles DO - 10.3329/ajmbr.v2i1.27574 UR - https://banglajol.info/index.php/AJMBR/article/view/27574 SP - 95-106 AB - <p>An efficient high frequency plant regeneration protocol through direct organogenesis was developed for <em>Sevia rebaudiana</em>. Shoot tips containing axillary buds were used as an explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.6% (w/v) agar supplemented with various concentrations of benzy-ladenine (BA), kinetin (Kn) and thidiazuron (TDZ).. BAP proved to be a better choice than Kn and the maximum number of shoots (3.75) was obtained on 2.0 mgL<sup>-1</sup> BAP concentration. Considering all parameters, combination of BAP and Kn gave comparatively better performance than single BAP or Kn. TDZ was effective for multiple shooting. Though, 1.5 mgL<sup>-1</sup> TDZ gave the best number of shoots (14.5), but 1.0 mgL<sup>-1</sup> TDZ gave best performance in response to all parameters under study. The highest number of shoots was obtained in 60 mlL<sup>-1</sup> coconut water, but 40 mlL<sup>-1</sup> coconut water gave the best result to all parameters. Root induction was tested by using two auxins namely NAA and IBA at different concentrations (1.0, 1.5, 2.0 mgL<sup>-1</sup>) on the MS medium. IBA at 1.0 mgL<sup>-1</sup> increased the rooting response (66.67%), number of roots (7.0) and root length (2.9 cm). Higher concentration of IBA and NAA (2.0 mgL<sup>-1</sup>) showed poor results of rooting response (33.33%).</p><p>Asian J. Med. Biol. Res. March <em>2016, 2(1): 95-106</em></p> ER -