Qualitative and quantitative analysis of buffalo ovaries, follicles and oocytes in view of in vitro production of embryos

Authors

  • MAMY Khandoker Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • N Jahan Istituto di Zootechnica, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, Piacenza 29122
  • L Asad Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • SAM Hoque Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • S Ahmed Bangladesh Livestock Research Institute, Savar, Dhaka 1341
  • MO Faruque Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202

DOI:

https://doi.org/10.3329/bjas.v40i1-2.10786

Keywords:

Buffalo, Corpus luteum, Follicles, Cumulus-oocyte-complexes

Abstract

This study was conducted to evaluate the buffalo ovaries, follicles and cumulus-oocyte-complexes (COCs) with the view of in vitro production (IVP) of embryos. Buffalo (Bubalis bubalis) ovaries were collected from local slaughterhouse and categorized according to the position of ovaries in the genital tract (left vs. right) and presence or absence of corpus luteum (CL). Moreover, collected ovaries were also evaluated for length, width and weight, number of follicles, aspirated follicles and state of COCs. The length (cm) of right ovary (2.32±0.06) was significantly (P<0.05) higher than left ones (2.14±0.05). Number of follicles were higher (P<0.05) in the left (7.25±0.31) compare to the right (6.22±0.32) ovaries. Other parameters, including width, weight and number of COC aspirated did not differ significantly (P<0.05) between right and left ovaries. The length, width and weight of ovaries with CL were higher (P<0.05) whereas, number of observed follicles, aspirated follicles, number of COCs and number of normal COCs were significantly (P<0.05) higher in ovaries without CL than their counter parts.

DOI: http://dx.doi.org/10.3329/bjas.v40i1-2.10786

Bang. J. Anim. Sci. 2011. 409 (1-2): 23-27

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Published

2012-05-28

How to Cite

Khandoker, M., Jahan, N., Asad, L., Hoque, S., Ahmed, S., & Faruque, M. (2012). Qualitative and quantitative analysis of buffalo ovaries, follicles and oocytes in view of in vitro production of embryos. Bangladesh Journal of Animal Science, 40(1-2), 23–27. https://doi.org/10.3329/bjas.v40i1-2.10786

Issue

Vol. 40 No. 1-2 (2011)

Section

Articles

License

© Bangladesh Journal of Animal Science.

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