@article{MAMY_Atiqah_Ariani_2017, title={Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow}, volume={45}, url={https://banglajol.info/index.php/BJAS/article/view/31034}, DOI={10.3329/bjas.v45i3.31034}, abstractNote={<p>This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus-oocyte-complexes (COCs) and to compare the effect of collection techniques on the recovery rate of COCs.  The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups.  It was found that 62.5% of the ovaries collected were CL- type and 37.5% were CL+ type.  For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded.  The higher number of follicles were dissected and aspirated from CL - ovaries (11.2±1.8and 37.8±14.9, respectively) compared to CL+ ovaries 10.5±1.5and 28.3±15.6, respectively).  The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells).  Grade A and grade B were classified as normal and grade C and grade D were considered as abnormal COCs.  The result indicated that ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique.  But same trend of result was not found in aspiration technique.  Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal basis) technique.  So, ovaries without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the <em>in vitro </em>embryo production experiment.</p><p class="yiv2013906861msonormal"><strong><em>Bang. J. Anim. Sci. 2016. 45 (3): </em></strong><strong><em>10-16</em></strong></p>}, number={3}, journal={Bangladesh Journal of Animal Science}, author={MAMY, Khandoker and Atiqah, NF and Ariani, N}, year={2017}, month={Jan.}, pages={10–16} }