@article{Roy_Moniruzzaman_Pasha_Modak_Alam_Islam_Khatun_Sarker_Kabir_Das_2022, title={PGF<sub>2α</sub> induced estrus synchronization of native buffaloes in Bangladesh}, volume={50}, url={https://banglajol.info/index.php/BJAS/article/view/58134}, DOI={10.3329/bjas.v50i2.58134}, abstractNote={&lt;p&gt;Buffaloes have low fertility due to silent estrus, seasonal anestrous, delayed puberty and long calving interval. This study was aimed to investigate the effect of single and double doses of prostaglandin F&lt;sub&gt;2alpha&lt;/sub&gt; (PGF2&lt;sub&gt;a&lt;/sub&gt;) on synchronization of estrus in buffaloes. A total of 66 buffaloes were selected and subjected to estrus synchronization experiments: single dose PGF2&lt;sub&gt;a&lt;/sub&gt;, and double dose PGF2&lt;sub&gt;a&lt;/sub&gt;. In single dose PGF2&lt;sub&gt;a&lt;/sub&gt; experiment, buffaloes were injected with either 500 or 875 µg of cloprostenol (PGF2&lt;sub&gt;a&lt;/sub&gt;) and all buffaloes were examined to identify corpus luteum through rectal palpation before administration of PGF2&lt;sub&gt;a&lt;/sub&gt;. In double dose experiment, buffaloes were injected with either 500 or 875 µg of cloprostenol (PGF2&lt;sub&gt;a&lt;/sub&gt;) on Day 0 and Day 12 without identifying corpus luteum. The buffaloes were examined for estrus by collecting cervical mucus using universal artificial insemination gun from the mid cervix and timed artificial inseminations (TAI) were done after 72 hrs of last dose of PGF2&lt;sub&gt;α&lt;/sub&gt; injection. The results showed that there were no significant differences in estrus rate (75±3.96% and 85±0.79%) single dose of PGF2&lt;sub&gt;α&lt;/sub&gt; with 500 and 875 µg of cloprostenol, respectively. However, 875 µg of cloprostenol significantly (P&amp;lt;0.05) increased estrus rate in buffaloes than double doses with 500 and 875 µg of cloprostenol (60 ± 12.72% and 63 ± 2.22%). The conception rate in buffaloes were 45±6.91% and 55±6.91% in 500 µg and 875 µg of cloprostenol in single dose of PGF2&lt;sub&gt;a&lt;/sub&gt; and 30±14.69% and 37±8.81% in 500 µg and 875 µg of cloprostenol in double dose of PGF2&lt;sub&gt;a&lt;/sub&gt;. The calving rate in buffaloes were 10±5.20% and 15±0.79% in 500 µg and 875 µg of cloprostenol in single dose of PGF2&lt;sub&gt;a&lt;/sub&gt; and 10±8.33 and 12±6.18% in 500 µg and 875 µg of cloprostenol in double dose of PGF2&lt;sub&gt;a&lt;/sub&gt;. However, there were no significant differences in conception and calving rates in buffaloes neither between single and double doses PGF2&lt;sub&gt;a&lt;/sub&gt; nor different concentrations of PGF2&lt;sub&gt;a&lt;/sub&gt;. In conclusion, single dose of PGF2&lt;sub&gt;a&lt;/sub&gt; with 875 µg of cloprostenol injection efficiently increased estrus synchronization rate in buffaloes.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;&lt;em&gt;Bang. J. Anim. Sci. 2021. 50 (2):&lt;/em&gt;&lt;/strong&gt;&lt;strong&gt;&lt;em&gt; 80-85&lt;/em&gt;&lt;/strong&gt;&lt;/p&gt;}, number={2}, journal={Bangladesh Journal of Animal Science}, author={Roy, BC and Moniruzzaman, M and Pasha, MH and Modak, AK and Alam, MH and Islam, MN and Khatun, A and Sarker, MB and Kabir, AKMA and Das, SK}, year={2022}, month={Feb.}, pages={80–85} }