In vivo Anticancer Activity on Ehrlich Ascites Carcinoma (EAC) Cells and in vitro Antimicrobial Activity of Psidium guajava Bark Extracts

Authors

  • Md Jakir Hossain Department of Biochemistry and Microbiology, North South University, Dhaka 1229
  • Shashwata Biswas Department of Microbiology, University of Dhaka, Dhaka 1000
  • Mohammad Shahriar Department of Pharmacy, University of Asia Pacific, Dhaka 1215
  • Sohidul Islam Department of Biochemistry and Microbiology, North South University, Dhaka 1229
  • Chowdhury Rafiqul Ahsan Department of Pharmacy, University of Asia Pacific, Dhaka 1215

DOI:

https://doi.org/10.3329/bjm.v35i1.39808

Keywords:

Psidium guajava, anticancer activity, antimicrobial activity

Abstract

This study was performed to evaluate the in vivo anticancer activity against ehrlich ascites carcinoma (EAC) cells and in vitro antimicrobial activity of Psidium guajava bark extracts. By soxhlet apparatus, the P. guajava bark extracts were obtained using four solvents (n-hexane, petroleum benzene, chloroform, and methanol) according to their increasing solubility. In case of in vivo anticancer activity of the sample extracts, mice were seeded with approximately 1x105 ehrlich ascites carcinoma (EAC) cells. After seven days of consecutive treatment, the negative and positive control groups (n=8 each group) showed an average EAC cell count of 2.4x108 and 1.8x108 respectively, and the experimental groups showed the cell count of 2.2x 108, 2.1x108, 1.9x108, and 1.41x108 when mice received h-hexane, petroleum benzene, chloroform, and methanol extract respectively. Experimental group that received methanol extract showed percent increase of life span (% ILS) of 33.3 when compared with the negative control. However, treatment in a cyclic manner of the mice showed % ILS of 52.15 for experimental group when compared negative control. In antimicrobial activity experiment, an intermediate zone of sensitivity of the crude methanol extract was found against Escherichia coli, Shigella flexneri, and Staphylococcus aureus when compared with amoxicillin. All these results indicated the anticancer activity and antimicrobial activity of the methanol extract of P. guajava barks on different experimental models.

Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 79-81

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Author Biographies

Md Jakir Hossain, Department of Biochemistry and Microbiology, North South University, Dhaka 1229

Department of Biochemistry and Microbiology

Shashwata Biswas, Department of Microbiology, University of Dhaka, Dhaka 1000

Department of Microbiology

Mohammad Shahriar, Department of Pharmacy, University of Asia Pacific, Dhaka 1215

Department of Pharmacy

Sohidul Islam, Department of Biochemistry and Microbiology, North South University, Dhaka 1229

Department of Biochemistry and Microbiology

Chowdhury Rafiqul Ahsan, Department of Pharmacy, University of Asia Pacific, Dhaka 1215

Department of Pharmacy

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Published

2019-01-15

How to Cite

Hossain, M. J., Biswas, S., Shahriar, M., Islam, S., & Ahsan, C. R. (2019). In vivo Anticancer Activity on Ehrlich Ascites Carcinoma (EAC) Cells and in vitro Antimicrobial Activity of Psidium guajava Bark Extracts. Bangladesh Journal of Microbiology, 35(1), 79–81. https://doi.org/10.3329/bjm.v35i1.39808

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Section

Short Communications