Role of Chitin for Harbouring of Toxigenic <i>Vibrio cholerae</i> O1 El Tor in Aquatic Environment

Authors

  • M Mahmud Hasan DNA Division, National Institute of Biotechnology (NIB), Ganakbari, Savar, Dhaka
  • Sucharit Basu Neogi Laboratory Science Division (LSD), International Centre for Diarrhoeal Diseases & Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka
  • Iqbal Kabir Jahid Laboratory Science Division (LSD), International Centre for Diarrhoeal Diseases & Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka
  • M Sirajul Islam Laboratory Science Division (LSD), International Centre for Diarrhoeal Diseases & Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka
  • Anwara Begum Department of Zoology, University of Dhaka, Dhaka

DOI:

https://doi.org/10.3329/bjm.v25i1.4851

Keywords:

Vibrio cholerae, Chitin, Polymerase chain reaction (PCR), Microcosm

Abstract

Vibrio cholerae, the etiological agent of cholera is an autochthonous bacterium of aquatic environment, often found attached to crustacean zooplankton. Chitin is the main component of crustacean exoskeleton, an insoluble polysaccharide. V. cholerae can secrete chitinase enzymes, which can facilitate the bacterium's association with chitinous aquatic organisms to utilize it as a potential nutrient source. In the present study, we checked the role of chitin for long term survival of V. cholerae. Laboratory based microcosms were prepared with purified artificial chitin chips and a toxigenic strain of V. cholerae O1 El Tor. In the presence of chitin, V. cholerae was found in a higher cellular density for >61 days in culturable condition than control water. We observed that the older chitin chips gradually thinner which indicated that V. cholerae utilized chitin. PCR experiment confirmed the presence of the bacterium's cholera toxin coding gene (ctxA) and membrane associated virulent gene ompU remained unaltered. Finally, from randomly amplified polymorphic DNA (RAPD) analysis no significant genotypic variation was detected in the bacterium's genome after its long time association with chitin.

Keywords: Vibrio cholerae; Chitin; Polymerase chain reaction (PCR); Microcosm

DOI: http://dx.doi.org/10.3329/bjm.v25i1.4851

Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 26-30

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How to Cite

Hasan, M. M., Neogi, S. B., Jahid, I. K., Islam, M. S., & Begum, A. (2010). Role of Chitin for Harbouring of Toxigenic <i>Vibrio cholerae</i> O1 El Tor in Aquatic Environment. Bangladesh Journal of Microbiology, 25(1), 26–30. https://doi.org/10.3329/bjm.v25i1.4851

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