PhoP-PhoQ Two-Component System Required for Colonization Leading to Virulence of <i>Dickeya dadantii</i> 3937 <i>in planta</i>

Authors

  • M Manjurul Haque Department of Microbiology, Bangladesh Jute Research Institute (BJRI), Manik Mia Avenue, Sher-e-Bangla Nagar, Dhaka
  • Kamrun Nahar Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipu
  • M Abdur Rahim Department of Microbiology, Bangladesh Jute Research Institute (BJRI), Manik Mia Avenue, Sher-e-Bangla Nagar, Dhaka
  • Isidore Gomes Department of Microbiology, Bangladesh Jute Research Institute (BJRI), Manik Mia Avenue, Sher-e-Bangla Nagar, Dhaka
  • Shinji Tsuyumu Labortory of Plant Pathology, Graduate School of Science & Technology, Shizuoka University, 836 Ohya, Shizuoka

DOI:

https://doi.org/10.3329/bjm.v25i1.4853

Keywords:

Virulence, Bacterial population, Magnesium, Dickeya dadantii

Abstract

Previously, we have reported that the PhoP-PhoQ two-component system (TCS) is essential for synthesis of pectate lyase, resistance to antimicrobial peptide and accumulation of acetyl-coenzyme A in Dickeya dadantii 3937. In this study, marker-exchange mutagenesis of phoP and phoQ of D. dadantii 3937 resulted in loss of virulence in Chinese cabbage and a severe reduction of bacterial population in infected area and its surrounding healthy tissue was also seen. Inoculation of bacteria grown and re-suspended in medium containing low concentration of Mg2+ resulted in reduced virulence and significantly reduced bacterial population in wild type while these mutants showed higher virulence and higher bacterial populations at high Mg2+ condition. Since Mg2+ concentration was detected to be very low (14.6 ± 4.23/100 g) in the apoplastic fluids of Chinese cabbage thus, the PhoP-PhoQ two-component system of D. dadantii 3937 is compromised virulence, at least in part, due to colonization of bacteria in planta responding to Mg2+.

Keywords: Virulence; Bacterial population; Magnesium, Dickeya dadantii

DOI: http://dx.doi.org/10.3329/bjm.v25i1.4853

Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 36-40

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How to Cite

Haque, M. M., Nahar, K., Rahim, M. A., Gomes, I., & Tsuyumu, S. (2010). PhoP-PhoQ Two-Component System Required for Colonization Leading to Virulence of <i>Dickeya dadantii</i> 3937 <i>in planta</i>. Bangladesh Journal of Microbiology, 25(1), 36–40. https://doi.org/10.3329/bjm.v25i1.4853

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