Effect of Radiation Sterilization on the Physicochemical Properties and Microbial Load of Amoxicillin Tri-Hydrate Powder

Authors

  • M Humayun Kabir Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Anisur Rahman Khan Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Marufa Zerin Akhter Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • M Abdul Malek Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Nazmul Alam Chowdhury Institute of Food & Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), PO Box 3787, Dhaka 1000, Bangladesh
  • Naiyyum Choudhury Atomic Energy Commission (BAEC), 4 Kazi Nazrul Islam Avenue, Dhaka 1000, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v23i2.886

Keywords:

Amoxicillin tri-hydrate, Irradiation, Radiosterilization

Abstract

Amoxicillin tri-hydrate powder was irradiated at different doses of gamma radiation, and the subsequent changes in terms of physicochemical properties and potency were evaluated. A dose of 0.5 Mrad was found to be enough to sterilize the powder, which had an initial bacterial count of 6.2 x 101 cfu/ml. The original snow-white colour of the powder changed to light brown upon irradiation doses over 2.5 Mrad. The pH decreased upon increase of radiation dose. The iodometric titration assay showed only a little variation in potency (about 1-4%) of the test materials after irradiation. High performance liquid chromatography (HPLC) analysis of irradiated amoxicillin tri-hydrate powder showed no marked loss of potency by irradiation even up at a dose of 10.0 Mrad. No degradation product and extra peak was found at this high dose. The results indicated that dried powder of amoxicillin tri-hydrate is stable after sterilization by irradiation.

Keywords: Amoxicillin tri-hydrate, Irradiation, Radiosterilization

DOI: http://dx.doi.org/10.3329/bjm.v23i2.886

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 165-167

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How to Cite

Kabir, M. H., Khan, A. R., Akhter, M. Z., Malek, M. A., Chowdhury, N. A., & Choudhury, N. (2008). Effect of Radiation Sterilization on the Physicochemical Properties and Microbial Load of Amoxicillin Tri-Hydrate Powder. Bangladesh Journal of Microbiology, 23(2), 165–167. https://doi.org/10.3329/bjm.v23i2.886

Issue

Vol. 23 No. 2 (2006)

Section

Short Communications