Evaluation of indirect immunofluroscence on HEP-2 cell and enzyme immunoassay methods for detection on antinuclear antibodies

Abstract

The suspicion of autoimmune disease primarily starts with clinical symptoms. The ELISA and IIF (indirect immunofluorescence) on HEp-2 cell   methods are comparable for detecting ANA in patient with auto immune diseases (AID). 102 patients attending Rheumatology out  patient  department as new or old cases with provisional diagnosis of SLE, RA, MCTD  and other Connective Tissue disorder (CTD) screened for specific autoantibody by ELISA (anti CCP, ANA and anti dsDNA) were subjected to indirect immunofluorescence on HEp-2 cell line. Diagnostic accuracy  based on ELISA and indirect immunoflurescence on HEp-2 cell line  showed  the  results of sensitivity, specificity,  PPV, NPV, accuracy of ANA were found to be 78.9%, 33.3%, 78.9%, 33.3%. 68%  respectively.  Sensitivity, specificity, PPV, NPV and the accuracy of anti-dsDNA   was 80%, 40%, 80%.40% and 70% respectively. Sensitivity, specificity, PPV, NPV, and the accuracy of anti CCP was found to be 64.3%, 72.2%, 64.3%, 72.2% and 68.8% respectively. The results indicates there is a need for screening all suspected autoimmune patient by indirect immunofluorescence on HEp-2 cell line before going to specific test.

DOI: http://dx.doi.org/10.3329/bjmm.v7i1.19315

Bangladesh J Med Microbiol 2013; 07(01): 11-14