Bacteriological profile and antibiotic susceptibility patterns of wound infections at Uttara Adhunik Medical College Hospital, Dhaka

Abstract

Background and objectives: Wound infection is one of the most important causes of morbidity and mortality worldwide and antibiotic resistant bacteria are a great part of complications in treatment of the infection. The present study was conducted to isolate and identify the etiological agents of wound infection and to assess the antimicrobial susceptibility pattern of the isolates.

Methodology: The retrospective study was carried out on wound infection suspected patients for six months duration. The collected pus specimens were first observed macroscopically then streaked on MacConkey agar, blood agar and incubated at 35°C for 24 to 48 hours. The isolated bacteria were identified by macroscopic and microscopic observations and biochemical reactions. Antibiotic susceptibility pattern of the isolates were assessed by Modified Kirby Bauer disc diffusion technique.

Results: A total of 146 pus samples were collected; of which 84 (57.5%) showed bacterial growth. Out of a total 84 bacterial isolates; 47 (55.95%) were gram negative and 37 (44.05%) were gram positive bacteria. Staphylococcus aureus (42.86%) was the most common bacteria followed by Pseudomonas spp. (25%), Escherichia coli (15.48%), Klebsiella pneumoniae (5.95%), Proteus spp (4.76%), Enterobacter spp (3.57%) and Streptococcus spp. (1.19%). S.aureus isolates were sensitive to Linezolid (100%), Rifampicin (100%), Doxycycline (86.11%), Clindamycin (83.33%) and Cloxacillin (75%). Low sensitivity of S.aureus to Ciprofloxacin (33.33%) and Azithromycin (33.33%). Among gram negative isolates Pseudomonas aeruginosa were found highly sensitive to Imipenem (93.54%), Piperacillin-Tazobactum (87.09%), Cefepime (80.64%) and Amikacin (77.42%). Low sensitivity of Pseudomonas aeruginosa to Aztreonam (35.48%) and Ceftazidime (51.61%). Isolated Escherichia coli were highly sensitive (98%) to Imipenem, Amikacin and showed lowest sensitivity to almost all of the other drugs.

Conclusion: By this study it is recommended, culture of wound swab and antibiotic susceptibility testing should be done before starting antibiotics, which will guide medical practitioners for empirical treatment of wound infection, so as to reduce the spread of drug resistant bacteria.

Bangladesh J Med Microbiol 2020; 14 (1): 15-19