Xpert MTB/RIF assay among the extra-pulmonary and non-sputum respiratory samples: One year experience at National Tuberculosis Reference Laboratory, Bangladesh

Adeeba Khanduker; Sadia   Sharmin; Shamim   Hossain; Tanvir   Huda; Farzana Mahzabin   Monika; Elma   Mostofa; S M Mostofa   Kamal; Afroza   Begum; Md Roknuzzaman   Khan; S M Abdur   Razzaque; Pronab Kumar   Modak; Md Shirajul   Islam

doi:10.3329/bjmm.v17i1.68139

Authors

Adeeba Khanduker Lecturer, Department of Microbiology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0000-0001-6442-7095
Sadia Sharmin Associate Professor, Department of Microbiology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0000-0001-5659-9890
Shamim Hossain Medical Technologist (Laboratory), National Tuberculosis Reference Laboratory, Dhaka https://orcid.org/0009-0002-5046-0008
Tanvir Huda Microbiologist, National Tuberculosis Reference Laboratory, Dhaka https://orcid.org/0009-0005-1749-0290
Farzana Mahzabin Monika Lecturer, Department of community Medicine, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0009-0007-6902-8828
Elma Mostofa Lecturer, Department of Pharmacology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0009-0004-7817-8711
S M Mostofa Kamal Professor & Head, Department of Microbiology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0000-0003-4439-0752
Afroza Begum Associate Professor, Department of Pathology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0009-0007-7549-5799
Md Roknuzzaman Khan Medical Technologist, Department of Microbiology, Anwer Khan Modern Medical College, Dhaka https://orcid.org/0009-0006-8215-8646
S M Abdur Razzaque Associate Professor, Department of Respiratory Medicine, National Institute of Diseases of the Chest and Hospital, Dhaka https://orcid.org/0009-0005-9249-0135
Pronab Kumar Modak Project Manager, National Tuberculosis Control Programme, Dhaka https://orcid.org/0009-0001-3885-0284
Md Shirajul Islam Assistant Professor (Resident Physician), National Institute of Diseases of the Chest and Hospital, Dhaka https://orcid.org/0009-0001-4741-1566

Keywords:

EPTB; Non-sputum respiratory samples; Xpert MTB/RIF assay; National Tuberculosis; Reference Laboratory

Abstract

Background: Extra-pulmonary tuberculosis (EPTB) is a diagnostic and therapeutic challenge due to the paucity of suitable rapid diagnostic tools.

Objectives: The aim was to assess the frequency of detection of Mycobacterium tuberculosis using Xpert MTB /RIF assay among the extra-pulmonary and non-sputum respiratory samples (NSRS) along with demographic profile, life style variables and proportion of Rifampicin resistant TB among those specimens.

Methodology: This cross-sectional retrospective study was conducted at National Tuberculosis Reference Laboratory (NTRL) from January 2018 to December 2018 for period of 1 year. Total 3918 extra-pulmonary and non-sputum respiratory samples from suspected TB patients were enrolled. Detection of Mycobacterium tuberculosis and Rifampicin resistance was performed by Xpert MTB /RIF assay and culture in both liquid and solid media.

Results: Among 3918 samples, 3152 were new cases and 766 were previously treated TB cases. A total of 853 (21.77%) MTB were detected by Xpert MTB /RIF assay. Among them, 713 (83.59%) were extra-pulmonary and 140 (16.41%) were non-sputum respiratory samples. Lymph node (LN) aspirates and tissues were the most frequent (37.9 and 9.9%) samples of EPTB. The proportion of RR cases among EPTB was 2.8% and 6.3% among new and previously treated cases. And 2.6% and 11.1% among NSRS new and previously treated cases respectively.

Conclusion: This study will give an insight of EPTB and NSRS profile with a view to diagnostic and therapeutic approach in Bangladesh.

Bangladesh Journal of Medical Microbiology, January 2023;17(1):21-28

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Xpert MTB/RIF assay among the extra-pulmonary and non-sputum respiratory samples: One year experience at National Tuberculosis Reference Laboratory, Bangladesh

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