Prevalence of Autoantibodies in Healthy Adults in Pakistani Population

Authors

  • Sara Ashraf Department of Immunology, University of Health Sciences Lahore
  • Nadeem Afzal Professor of Immunology, Department of Immunology, University of Health Sciences Lahore
  • Muhammad Kashif Doctoral Research Fellow, Department of Immunology University of Health Sciences Lahore
  • Faheem Shahzad Doctoral Research Fellow, Department of Immunology University of Health Sciences Lahore
  • Wasif Sajjad Department of Immunology, University of Health Sciences Lahore,
  • Waqas Latif Biostatician, University of Health Sciences Lahore
  • Afia Abbas Senior Lab Manger, Lahore

DOI:

https://doi.org/10.3329/bjms.v15i4.27834

Keywords:

ANAs, Anti dsDNA, Anti RF, Autoantibodies, Autoimmune diseases

Abstract

Introduction: Autoimmune phenomenon is attributed to a number of diseases which were once considered idiopathic. In humans, production of auto antibodies (a-Abs) against self-antigens is quite frequent but earlier their presence was associated with autoimmune diseases, however a-Abs have been documented in non-autoimmune disorders i.e. complicated pregnancy, cancer, stroke etc. In Pakistan, limited data is available on frequency of a-Abs, therefore this study was designed to determine serum level of antinuclear antibody (ANA), Rheumatoid factor (RF) and anti dsDNA antibodies in apparently healthy population.

Materials and methods: After written informed consent, blood sample of 256 subjects was obtained by random sampling. Participants of established autoimmune diseases were excluded. Enzyme linked immunosorbent assay (ELISA) was used to determine ANA, RF and anti-dsDNA antibody. Categorical variables were compared by using c2 test. A p value <0.05 was considered statistically significant.

Results: Rheumatoid factor was the most frequent a-Ab (18%), followed by anti dsDNA (7.4%), while ANA was the lowest (0.4%) antibody detected. Only RF had a statistically significant association with gender (p=0.047). No association of these antibodies with age was detected.

Conclusion: Rheumatoid factor auto antibody was more prevalent as compared to ANA and dsDNA antibody in healthy adults.

Bangladesh Journal of Medical Science Vol.15(4) 2016 p.579-582

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Published

2016-12-18

How to Cite

Ashraf, S., Afzal, N., Kashif, M., Shahzad, F., Sajjad, W., Latif, W., & Abbas, A. (2016). Prevalence of Autoantibodies in Healthy Adults in Pakistani Population. Bangladesh Journal of Medical Science, 15(4), 579–582. https://doi.org/10.3329/bjms.v15i4.27834

Issue

Vol. 15 No. 4 (2016)

Section

Original Articles

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