D-erythro-Sphingosine and Pregnenolonesulphate activate TRPM3 channels synergistically in INS- 1E cells

Authors

  • Md Abdul Halim Uppsala University, Department of Medical Sciences, Gastroenterology and Hepatology Unit,Rudbeck Lab, Hus R3, plan 4 (BV) Dag Hammarskjöldsväg 20, 751 85 Uppsala

DOI:

https://doi.org/10.3329/bjms.v16i1.31140

Abstract

Background: A group of ion channels have recently been studied to understand the pathogenesis of diabetes. The transient receptor potential (TRP) channels are thought to be involved in many cellular functions. TRPM3, a member of the melastatin-like transient receptor is mainly expressed in human kidney and brain. It is also expressed in human pancreas. Therefore, it is desirable to find compounds able to induce an increase of intracellular calcium([Ca2+]i) in pancreatic ? cells thereby trigger insulin secretion.

Aims: The aim of the study was to confirm whether D-erythro- Sphingosine and Pregnenolonesulphate activates TRPM3. Another aim was to investigate whether pancreatic ? cells express TRPM3-channels. INS-1E cells were used as a model of ?cells for [Ca2+]i measurement.

Results: Application of endogenous neurosteroidpregnenolonesulphate (35 µM) led to a rapid Ca2+ influx in INS-1E cells and pancreatic beta cells. When PS was applied in the absence of extracellular Ca2+ the [Ca2+]i response to PS was completely lost. The increase in [Ca2+]i induced by PS was inhibited by cholesterol.Western blot data identified a protein reacting specifically with polyclonal antibodies for TRPM3.

Conlusion: Our results demonstrate that both pancreatic ?cells and INS-1E cells express functional TRPM3-channels and both SPH and PS are TRPM3 agonists.

Bangladesh Journal of Medical Science Vol.16(1) 2017 p.98-106

Downloads

Download data is not yet available.
Abstract
5
PDF
8

Author Biography

Md Abdul Halim, Uppsala University, Department of Medical Sciences, Gastroenterology and Hepatology Unit,Rudbeck Lab, Hus R3, plan 4 (BV) Dag Hammarskjöldsväg 20, 751 85 Uppsala



Downloads

Published

2017-01-16

How to Cite

Halim, M. A. (2017). D-erythro-Sphingosine and Pregnenolonesulphate activate TRPM3 channels synergistically in INS- 1E cells. Bangladesh Journal of Medical Science, 16(1), 98–106. https://doi.org/10.3329/bjms.v16i1.31140

Issue

Vol. 16 No. 1 (2017)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.