Dual Signaling of the Fas Receptor: on Saudi Patients with Chronic Leukemia

Authors

  • Faris Q Alenzi College of Appl.Med.Sci, Prince Sattam bin Abdulaziz University, Al-Kharj
  • Fozah SM Alonizi College of Appl.Med.Sci, King Saud University, Riyadh
  • Amani A Ballow MD Labs, AlHabib Hospital
  • Meaad Q Alanazi Dept of Nursing, KAMC, Riyadh
  • Dalal S Al Shaya Dept of Biology, College of Science, PNU, Riyadh
  • Fahad G Alanazi MOH Hospitals, Riyadh
  • Omar Bagader MOH Hospitals, Riyadh
  • Abdulrahman Q Alanazi MOH Hospitals, Riyadh

DOI:

https://doi.org/10.3329/bjms.v16i1.31143

Abstract

Introduction: Purifed CD34+ cells and cultured colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were utilized to examine the role of Fas/FasL and caspase-8interactionsin the regulation of myelopoiesis.

Methods: Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RTPCR and immunofluorescence in each case. The functional tests for the CFU-GM were the standard settlement test and the proliferative limit of CFU-GM was measured by replating the essential cells and monitoring auxiliary colony development.

Results: Treatment of CFU-GM with IETD significantly increased, the proliferative limit, while the Fas mAb resulted in decreased the Fas and FasL expression were measuredutilizing RT-PCR and immunofluorescence individually.

Conclusion: Fas, FasL, and caspase attenuationplay important rolesin the control of myelopoiesis.

Bangladesh Journal of Medical Science Vol.16(1) 2017 p.122-126

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Author Biography

Faris Q Alenzi, College of Appl.Med.Sci, Prince Sattam bin Abdulaziz University, Al-Kharj



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Published

2017-01-16

How to Cite

Alenzi, F. Q., Alonizi, F. S., Ballow, A. A., Alanazi, M. Q., Shaya, D. S. A., Alanazi, F. G., Bagader, O., & Alanazi, A. Q. (2017). Dual Signaling of the Fas Receptor: on Saudi Patients with Chronic Leukemia. Bangladesh Journal of Medical Science, 16(1), 122–126. https://doi.org/10.3329/bjms.v16i1.31143

Issue

Vol. 16 No. 1 (2017)

Section

Original Articles

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