Comparative studies evaluating macrophage activity of pulmonary tuberculosis patients with and without diabetes mellitus (tb-dm and non tb-dm)

Authors

  • Antari Arlita Leniseptaria Department of Microbiology Faculty of MedicineDiponegoro University
  • Indah Saraswati Department of Chemistry Faculty of Medicine Diponegoro University
  • David Pakaya Department of Histology Faculty of Medicine and Health Science Tadulako University

DOI:

https://doi.org/10.3329/bjms.v17i1.35290

Keywords:

macrophage, TB-DM, phagocytosis, intracellular killing, IFN-γ.

Abstract

Background: Macrophages, as the first defense mechanism in Mycobacterium tuberculosis infection, play the important role in pulmonary TB pathogenesis. The increasing prevalence of pulmonary TB is followed by the increasing prevalence of diabetes mellitus (DM). DM patients have 4,7 times higher risk to develop pulmonary TB compared to patients without DM, since DM can increase the frequency and severity of an infection, including pulmonary TB.

Aim: To analyze macrophage activity (phagocytosis, intracellular killing, and IFN-γsynthesis) of TBDM and TB non-DM patient.

Method: This experimental study used a PBMC cultured sample from TB-DM and TB non-DM patient’s which undergo observation of macrophage activity (phagocytic, intracellular killing and IFN-γsynthesis). The data were taken from microscopic observation of TB-DM and TB non-DM patients, colony growth of viable M. tuberculosis and the IFN-γlevel secreted by macrophages.

Result: The result showed that macrophages of TB-DM patient’s were less amount of phagocytosedM. tuberculosis, a little amount of formed vacuoles and giant cells, secrete low level of IFN-γ, and more viable M. tuberculosis(from subculture).

Conclusions: Macrophages of TB-DM patients are reduced phagocytic activity toward M. tuberculosis which is this macrophages are less activated.

Bangladesh Journal of Medical Science Vol.17(1) 2018 p.107-111

Downloads

Download data is not yet available.
Abstract
13
PDF
23

Downloads

Published

2018-01-11

How to Cite

Leniseptaria, A. A., Saraswati, I., & Pakaya, D. (2018). Comparative studies evaluating macrophage activity of pulmonary tuberculosis patients with and without diabetes mellitus (tb-dm and non tb-dm). Bangladesh Journal of Medical Science, 17(1), 107–111. https://doi.org/10.3329/bjms.v17i1.35290

Issue

Vol. 17 No. 1 (2018)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.