Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro
DOI:
https://doi.org/10.3329/bjms.v17i4.38315Keywords:
ethanolic garlic extract, human gingival fibroblast, in vitro, biocompatibility, proliferationAbstract
Introduction: Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory effect. Moreover, garlic extract has a good biocompatibility and can stimulate cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied.
Objective: The aim of this study was to analyze the biocompatibility of garlic extract by observing the viability and proliferation of human gingival fibroblasts in vitro.
Methods: Biocompatibility test was conducted using serial concentration of garlic extract. Human gingival fibroblasts was seeded into 96 microwell plate with density of 2x103 cells, added with the fourteen serial concentration of garlic extract, and incubated in 37o C and 5% CO2for 24, 48 and 72 hours. MTT assay was used to analyze the viability and proliferation of human gingival fibroblasts. Data were analyzed by the Kruskal Wallis and U Mann-Whitney test.
Results: The result showed that in each time of observation, there is no significant difference in viability fibroblast (p>0,05), but there are significant difference between time of observation at 24, 48, and 72 hours (p <0.05).Data showed that all concentration of garlic extract increased the viability and proliferation of human gingival fibroblasts.
Conclusions: The ethanolic garlic extract has a good biocompatibility to human gingival fibroblasts culture cell and can stimulate the proliferation of human gingival fibroblast.
Bangladesh Journal of Medical Science Vol.17(4) 2018 p.556-561
Downloads
21
24
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:
- Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.
Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.- Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.