Association between polymorphism of lys198asn endothelin-1 gene and endothelin-1 plasma level in javanese obesity population

Authors

  • Dewi Karita Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia
  • Ahmad Hamim Sadewa Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia
  • Pramudji Hastuti Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada

DOI:

https://doi.org/10.3329/bjms.v18i1.39546

Keywords:

endothelin-1; obesity; polymorphism; vasoconstrictor; ET-1.

Abstract

Background: Endothelin-1 plays a role as a vasoconstrictor and also inhibits the expression of adiponectin in obesity. The Lys198Asn polymorphism of the endothelin-1 gene will increase the level of endothelin-1 plasma and increase the risk factor of obesity.

Objective: In this study, we investigated the relationship between Lys198Asn polymorphism of endothelin-1 gene and endothelin-1 plasma level among individuals with obesity in Javanese population.

Method: This study was conducted on 61 obese subjects and 65 control subjects. All subjects were measured for anthropometrics and endothelin-1 plasma level by ELISA (Enzyme-linked Immune Sorbent Assay). The polymorphism of Lys198Asn of ET-1 (Endothelin-1) was screened by using PCRRFLP (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism).

Results: TT genotype was a risk factor of obesity (OR 5.344 CI=95% 1.77-16.16) compared with GG genotype. Levels of endothelin-1 plasma were higher in obese subjects than that of control subjects (p=0.013), with TT genotype having the highest endothelin-1 plasma level (p<0.001).

Conclusion: The Lys198Asn polymorphism of endothelin-1 gene plays a role in increasing endothelin-1 plasma levels and is risk factors of obesity.

Bangladesh Journal of Medical Science Vol.18(1) 2019 p.46-49

Downloads

Download data is not yet available.
Abstract
20
PDF
18

Author Biographies

Dewi Karita, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia

Department Of Biochemistry

Ahmad Hamim Sadewa, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia

Department Of Biochemistry

Pramudji Hastuti, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada

Department Of Biochemistry

Downloads

Published

2018-12-30

How to Cite

Karita, D., Sadewa, A. H., & Hastuti, P. (2018). Association between polymorphism of lys198asn endothelin-1 gene and endothelin-1 plasma level in javanese obesity population. Bangladesh Journal of Medical Science, 18(1), 46–49. https://doi.org/10.3329/bjms.v18i1.39546

Issue

Vol. 18 No. 1 (2019)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.