Evaluation of Semen Samples Before and After ‘Swim Up’ Technique with Mitotracker

Authors

  • Seda Cetinkaya Karabekir Assistant Professor Seda Cetinkaya Karabekir, University of KTO Karatay, Faculty of Medicine, Department of Histology and Embryology, Fetih neighborhood, Alaaddin Kap Street. Number: 130 Konya, TR 42020, Turkey
  • Aydan Özgörgülü University of Necmettin Erbakan, Faculty of Meram Medicine, Department of Histology and Embryology, Konya, Turkey

DOI:

https://doi.org/10.3329/bjms.v18i3.41614

Keywords:

sperm,swim-up method; MitoTracker 580; oligospermia

Abstract

Objectives: Infertility problems are seen in nearly 15% of married couples, and almost 50% of these involve male infertility. The sperm mitochondrion plays an important role in sperm motility. The morphology of sperm, sperm preparation techniques, and the number and motility of sperm during insemination are important parameters that affect the rate of pregnancy with intrauterine insemination. The aim of the study was to observe the changes in sperm mitochondrial activities and numbers with the Mito Tracker Red 580 kit at 30th, 45th and 60th minutes after swim up.

Materials and Methods: In this study, we evaluated 20 oligospermic and 20 normospermic samples. The “swim-up” technique was applied to these oligospermic and normospermic samples, and changes in sperm mitochondria and number at 30, 45, and 60 min were observed using MitoTracker Red 580 staining.

Results:The numbers of swimming sperm in oligospermic and normospermic samples were higher at 30 min than at 45 and 60 min. No marked change was detected in the mitochondrial activities of swimming sperm in oligospermic and normospermic samples at 30, 45, and 60 min after the swim-up. Our findings demonstrate that the number of swimming sperm decreased with time, but no change was seen in the mitochondrial activity of the sperm by the swim-up technique.

Conclusion: According to findings, mitochondrial movement is not exactly related to the 30th, 45th and 60th minutes. Therefore, it is thought that it needs to be investigated in mechanisms other than mitochondrial activity.

Bangladesh Journal of Medical Science Vol.18(3) 2019 p.479-483

Downloads

Download data is not yet available.
Abstract
17
PDF
26

Downloads

Published

2019-05-30

How to Cite

Karabekir, S. C., & Özgörgülü, A. (2019). Evaluation of Semen Samples Before and After ‘Swim Up’ Technique with Mitotracker. Bangladesh Journal of Medical Science, 18(3), 479–483. https://doi.org/10.3329/bjms.v18i3.41614

Issue

Vol. 18 No. 3 (2019)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.