Experimental research of Harmine hydrochloride effect on internal organs

Authors

  • Galina U Zhanaidarova Karaganda State Medical University, Karaganda, Kazakhstan
  • Roza Zh Yessimova Karaganda State Medical University, Karaganda, Kazakhstan
  • Kulash T Nurseitova Karaganda State Medical University, Karaganda, Kazakhstan
  • Roza B Seidakhmetova Karaganda State Medical University, Karaganda, Kazakhstan
  • Leila I Arystan Karaganda State Medical University, Karaganda, Kazakhstan
  • Sergazy M Adekenov CEO JSC ISPH «Phytohimiya», Karaganda, Kazakhstan
  • Nurlan N Nauryzov Karaganda State Medical University, Karaganda, Kazakhstan
  • Bakhyt Kh Berikbaeva Karaganda State Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3329/bjms.v18i3.41635

Keywords:

preclinical studies, chronic toxicity, harmine hydrochloride capsules, morphological examination, internal organs.

Abstract

Objective. The purpose of thisstudy was to determine the chronic toxicity of the drug “Harmine hydrochloride, capsules” for preclinical evaluation of its safety.

Materials and methods.The experiment was performed on 48 CD-1 rats. Harmine hydrochloride was injectedto the animals intragastrically at doses of 2.5 mg / kg/per day, 5 mg / kg/per day, 9 mg / kg/per day, 10 mg / kg/per day for 3 months. After 3 months, the animals were withdrew from the experiment, internal organs (brain, heart, spleen, adrenals) were weighed and set in 10% neutral formalin. Histological specimenswere mounted in accordance with standard procedures.

Results and Discussion. All morphological, histopathological changes, in addition to mortality and bodyweight changes were recorded.Microtome cuts, 5 micrometers thick, were stained with hematoxylin and eosin.

Conclusion. As a result of the morphological study, there was no toxic effect of harmine hydrochloride at doses of 2.5 mg / kg, 5 mg / kg, 9 mg / kg and 10 mg / kg on the brain, at doses of 2.5 mg / kg and 5 mg / kg on the structure of the heart, spleen and adrenal glands. At doses of 9 mg / kg and 10 mg / kg, there is an initial toxic dose-dependent effect on the heart, spleen and adrenal glands.

Bangladesh Journal of Medical Science Vol.18(3) 2019 p.598-606

Downloads

Download data is not yet available.
Abstract
19
PDF
28

Downloads

Published

2019-05-30

How to Cite

Zhanaidarova, G. U., Yessimova, R. Z., Nurseitova, K. T., Seidakhmetova, R. B., Arystan, L. I., Adekenov, S. M., Nauryzov, N. N., & Berikbaeva, B. K. (2019). Experimental research of Harmine hydrochloride effect on internal organs. Bangladesh Journal of Medical Science, 18(3), 598–606. https://doi.org/10.3329/bjms.v18i3.41635

Issue

Vol. 18 No. 3 (2019)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.