Standardadization and skin irritation potential of herbal analgesic cream containing Nigella sativa seed oil

Authors

  • Tehmina Sohail Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Shazia Yasmeen Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Hina Imran Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Sadia Ferheen Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Atiq U Rehman Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Rashid Ali Khan Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan

DOI:

https://doi.org/10.3329/bjms.v19i1.43891

Keywords:

Nigella sativa HPLC; standardization; skin irritancy; rabbits; human volunteers

Abstract

Background & Objective: The aim of present study was to evaluate herbal analgesic cream containing Nigella sativa oil as an active ingredient, standardization of Nigella sativa oil and evaluation of irritation/sensitization potential of formulation.

Material & Method: For this, HPLC analysis of oil, skin irritation test on rabbits and patch testing on human skin was conducted. HPLC analysis using C-18 column, using an isocratic mobile phase of water: methanol (10:90) at flow rate of 1 ml/inactive ingredient: thymoquinone purified from the oil was found to be 1.42g (28.4 %). Safety assessment of analgesic cream was based on Primary Dermal Irritation Index (PDII) by Draize method.

Result: According to Draize standard scoring system of reactions PDII was found 0.04 which comes under the category of non irritant. Patch testing on human volunteers revealed that none of volunteers showed any sign of skin reactions.

Bangladesh Journal of Medical Science Vol.19(1) 2020 p.163-168

Downloads

Download data is not yet available.
Abstract
40
PDF
55

Downloads

Published

2019-11-03

How to Cite

Sohail, T., Yasmeen, S., Imran, H., Ferheen, S., Rehman, A. U., & Khan, R. A. (2019). Standardadization and skin irritation potential of herbal analgesic cream containing Nigella sativa seed oil. Bangladesh Journal of Medical Science, 19(1), 163–168. https://doi.org/10.3329/bjms.v19i1.43891

Issue

Vol. 19 No. 1 (2020)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.