Standardadization and skin irritation potential of herbal analgesic cream containing Nigella sativa seed oil

Authors

  • Tehmina Sohail Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Shazia Yasmeen Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Hina Imran Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Sadia Ferheen Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Atiq U Rehman Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan
  • Rashid Ali Khan Pharmaceutical Research Centre, PCSIR LABS COMPLEX Karachi, Pakistan

DOI:

https://doi.org/10.3329/bjms.v19i1.43891

Keywords:

Nigella sativa HPLC; standardization; skin irritancy; rabbits; human volunteers

Abstract

Background & Objective: The aim of present study was to evaluate herbal analgesic cream containing Nigella sativa oil as an active ingredient, standardization of Nigella sativa oil and evaluation of irritation/sensitization potential of formulation.

Material & Method: For this, HPLC analysis of oil, skin irritation test on rabbits and patch testing on human skin was conducted. HPLC analysis using C-18 column, using an isocratic mobile phase of water: methanol (10:90) at flow rate of 1 ml/inactive ingredient: thymoquinone purified from the oil was found to be 1.42g (28.4 %). Safety assessment of analgesic cream was based on Primary Dermal Irritation Index (PDII) by Draize method.

Result: According to Draize standard scoring system of reactions PDII was found 0.04 which comes under the category of non irritant. Patch testing on human volunteers revealed that none of volunteers showed any sign of skin reactions.

Bangladesh Journal of Medical Science Vol.19(1) 2020 p.163-168

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Published

2019-11-03

How to Cite

Sohail, T., Yasmeen, S., Imran, H., Ferheen, S., Rehman, A. U., & Khan, R. A. (2019). Standardadization and skin irritation potential of herbal analgesic cream containing Nigella sativa seed oil. Bangladesh Journal of Medical Science, 19(1), 163–168. https://doi.org/10.3329/bjms.v19i1.43891

Issue

Vol. 19 No. 1 (2020)

Section

Original Articles

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