Phytochemical compound and non-cytotoxicity effect of sting bee and stingless bee honey against normal human gingival cell lines

Authors

  • Siti Lailatul Akmar School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Moeez Ansari School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Zurairah Berahim School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Wan Nazatul Shima Shahidan School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

DOI:

https://doi.org/10.3329/bjms.v21i1.56343

Keywords:

Phytochemical; Honey; Cytotoxicity; Human gingival fibroblast

Abstract

Objective: Both honeybees (Apis spp.) and stingless bees (Trigona spp.) produce honeys which normally taken orally, have high nutritional and therapeutics value. Until recently, phytochemical comparison of both honey is still scarce and elucidating cytotoxicity effects on human gingival fibroblast cells (HGF) in oral cavity is of interest.

Materials and Methods: Kelulut honey (KH), acquired from the stingless bees and acacia honey (AH) from the sting bees honey samples were underwent GC-MS analysis to ascertain their composition. HGF were exposed to various concentrations of KH and AH from the lowest 0.015% to the highest 5% by MTT assay for 24h, 48h and 72h.

Results: GC-MS analysis determined various beneficial compounds such as flavonoids, furans, pyrans, levoglucosan and hydroxymethylfurfural from both of honey samples. MTT assay showed that the HGF cells demonstrated good viability up to percentages (v/v) as high as almost 2% in both honeys. The IC50 values for both honey for all time frames fall at above 2%.

Conclusion: Both honey showed good survivability of HGF cells up to 2% of concentration.

Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 158-164

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Published

2022-01-01

How to Cite

Akmar, S. L. ., Ansari, M. ., Berahim, Z. ., & Shima Shahidan, W. N. . (2022). Phytochemical compound and non-cytotoxicity effect of sting bee and stingless bee honey against normal human gingival cell lines. Bangladesh Journal of Medical Science, 21(1), 158–164. https://doi.org/10.3329/bjms.v21i1.56343

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Original Articles