Phytochemical compound and non-cytotoxicity effect of sting bee and stingless bee honey against normal human gingival cell lines

Authors

  • Siti Lailatul Akmar School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Moeez Ansari School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Zurairah Berahim School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Wan Nazatul Shima Shahidan School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

DOI:

https://doi.org/10.3329/bjms.v21i1.56343

Keywords:

Phytochemical; Honey; Cytotoxicity; Human gingival fibroblast

Abstract

Objective: Both honeybees (Apis spp.) and stingless bees (Trigona spp.) produce honeys which normally taken orally, have high nutritional and therapeutics value. Until recently, phytochemical comparison of both honey is still scarce and elucidating cytotoxicity effects on human gingival fibroblast cells (HGF) in oral cavity is of interest.

Materials and Methods: Kelulut honey (KH), acquired from the stingless bees and acacia honey (AH) from the sting bees honey samples were underwent GC-MS analysis to ascertain their composition. HGF were exposed to various concentrations of KH and AH from the lowest 0.015% to the highest 5% by MTT assay for 24h, 48h and 72h.

Results: GC-MS analysis determined various beneficial compounds such as flavonoids, furans, pyrans, levoglucosan and hydroxymethylfurfural from both of honey samples. MTT assay showed that the HGF cells demonstrated good viability up to percentages (v/v) as high as almost 2% in both honeys. The IC50 values for both honey for all time frames fall at above 2%.

Conclusion: Both honey showed good survivability of HGF cells up to 2% of concentration.

Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 158-164

Downloads

Download data is not yet available.
Abstract
35
PDF
65

Downloads

Published

2022-01-01

How to Cite

Akmar, S. L. ., Ansari, M. ., Berahim, Z. ., & Shima Shahidan, W. N. . (2022). Phytochemical compound and non-cytotoxicity effect of sting bee and stingless bee honey against normal human gingival cell lines. Bangladesh Journal of Medical Science, 21(1), 158–164. https://doi.org/10.3329/bjms.v21i1.56343

Issue

Vol. 21 No. 1 (2022)

Section

Original Articles

License

Copyright (c) 2022 Siti Lailatul Akmar, Moeez Ansari, Zurairah Berahim, Wan Nazatul Shima Shahidan

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.