PCR-based screening of MNS glycophorin variants in patients suspected of having anti-Mia

Authors

  • Siti Nazihahasma Hassan School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
  • Suharni Mohamad School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
  • Rosline Hassan Department of Hematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
  • Shuangshi Wei CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China
  • Noor Haslina Mohd Noor Department of Hematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
  • Wan Suriana Wan Ab Rahman School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

DOI:

https://doi.org/10.3329/bjms.v21i2.58078

Keywords:

MNS blood group system, MNS glycophorin variants, Anti-Mia, Polymerase chain reaction

Abstract

Background:MNS glycophorin variants such as glycoprotein (GP.) Mur, GP.Hop, GP.Bun, GP.HF, GP.Hut, and GP.Vw are known to carry multiple antigens, including Mia (MNS7). In East and Southeast Asians, an antibody to Mia is not only commonly found, but also clinically significant.

Purpose and Methods: In this study, deoxyribonucleic acid (DNA) samples from 47 patients suspected of having anti-Mia by serological screening were genotyped for GP.Mur, GP.Hop, GP.Bun, GP.HF, GP.Hut, and GP.Vw variants using polymerase chain reaction (PCR) assay.

Results: All patients were found to be negative for the presence of Mia-positive glycophorin variants.

Conclusions: The presence of anti-Mia cannot be excluded in patients studied, as genotyping shows that Mia-positive MNS glycophorin variants are not present.

Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 432-437

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Published

2022-02-25

How to Cite

Hassan, S. N. ., Mohamad, S. ., Hassan, R. ., Wei, S. ., Noor, N. H. M. ., & Ab Rahman, W. S. W. . (2022). PCR-based screening of MNS glycophorin variants in patients suspected of having anti-Mia. Bangladesh Journal of Medical Science, 21(2), 432–437. https://doi.org/10.3329/bjms.v21i2.58078

Issue

Vol. 21 No. 2 (2022)

Section

Original Articles

License

Copyright (c) 2022 Siti Nazihahasma Hassan,Suharni Mohamad, Rosline Hassan, Shuangshi Wei, Noor Haslina Mohd Noor, Wan Suriana Wan Ab Rahman

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