The Effect of Alfalfa (Medicago sativa) extract onthe number of macrophage, fibroblast, and amount of collagen in rat wound healing
DOI:
https://doi.org/10.3329/bjms.v22i1.61864Keywords:
alfalfa; macrophage; fibroblast; collagenAbstract
Background: Alfalfa extract has been shown to have anti-inflammatory activity in wound healing.
Objectives: This study aims to investigate the effect of Alfafa on the number of macrophages, fibroblast, and collagen in the rat wound healing.
Method: In this post-testonly control group design study, 50 Wistar rats were randomly divided into five groups. K0 (negative control) group was treated with ointment base. K1 (positive control) group was treated with gentamicin. P1, P2, and P3 groups were treated with 10%, 20%, and 30% alfalfa extract respectively. The termination of rats conducted on day 3 and day 7 post of first treatment (5 rats/group/period). On day 3, the number of macrophage and fibroblast were evaluated using Hematoxylin-Eosin staining. On day 7, the number of collagen was evaluated using Siriusred staining. The data were analyzed using one way ANOVA followed by Tukey’s post hoc test.
Result: The result, showed a significant difference in the number of macrophages between the negative control treated group and K1 groups. The number of fibroblasts was significantly different between the P3 group and the P1, K1 group. There was a significant difference in the number of collagens between negative control andtreated group, and between the positive control and P2, P3 group.
Conclusion: The administration of alfalfa extract at the dose of 20% can accelerate wound healing by increasing the number of fibroblasts and collagen fibers and decreasing number of macrophages.
Bangladesh Journal of Medical Science Vol. 22 No. 01 January’23 Page : 77-83
Downloads
69
71
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Yustiana Arie Suwanto, Chodidjah, Atina Hussaana
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:
- Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.
Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.- Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.