Evaluating Salivary Apelin Level among Chronic Periodontitis Patients, with and without Type 2 Diabetes Mellitus utilizing Enzyme‑Linked Immunosorbent Assay: A Comparative Study

Authors

  • Tanvi Hirani Research Scholor, Department of Periodontology, Gujarat University, Ahmedabad, Gujarat, and Department of Periodontology, Karnavati School of dentistry, Uvarsad, Gandhinagar, India
  • Md Ahsanul Haq Infectious Diseases Division, icddr,b, Mohakhali, Dhaka, Bangladesh
  • Neetha J Shetty Department of Periodontology and Implantology, Manipal College of Dental Sciences, MAHE, Mangalore, Karnataka 575001, India
  • Viral Patel Department of Periodontology and Implantology, College of Dental Science and Research Center, Gujarat University, Ahmedabad, Gujarat, India
  • Rahnuma Ahmad Department of Physiology, Medical College for Women and Hospital, Dhaka, Bangladesh
  • Susmita Sinha Department of Physiology, Khulna City Medical College and Hospital, Khulna, Bangladesh
  • Siddhartha Dutta Department of Pharmacology, All India Institute of Medical Sciences, Rajkot, Gujarat, India
  • Mainul Haque Unit of Pharmacology. Faculty of Medicine and Defence Health, Universiti Pertahanan Nasional Malaysia (National Defence University of Malaysia), Kem Perdana Sungai Besi, 57000 Kuala Lumpur, Malaysia
  • Santosh Kumar Department of Periodontology and Implantology, Karnavati School of Dentistry, Karnavati University, Gandhinagar, Gujarat, India

DOI:

https://doi.org/10.3329/bjms.v22i2.64987

Keywords:

Assessment, Salivary Apelin, Concentration, Chronic Periodontitis, Type 2 Diabetes Mellitus (T2DM), ELISA, Compare, Contrast

Abstract

Background: Periodontitis is a chronic inflammatory disorder mainly due to a decrease in the immune system caused by periodontal pathogens. The relationship of apelin among chronic generalized periodontitis (CGP) and type 2 diabetes mellitus (T2DM) has been reported. This study intends to assess the level of apelin in saliva from patients of healthy individuals, individuals with CGP, and CGP + T2DM, and evaluate the role of apelin adipokine in inflammation and insulin resistance. New treatment modalities can be invented using apelin in host modulation therapies.

Methods: The study comprised 300 patients that were enrolled according to their clinical findings and were divided into three groups chronic periodontitis (n=100), chronic periodontitis with T2DM (n=100), and healthy controls (n=100). Probing pocket depth (PPD), clinical attachment level (CAL), and salivary Apelin (AP) were obtained. With the help of the enzyme-linked immunosorbent assay (ELISA) kit, the salivary apelin level was measured. A statistical software package was used for the surveying of collected data.

Results: Group three[CGP+DM] shows the highest salivary apelin level compared to CGP patients without DM. It was 15.43±2.05ng/dl in CGP and 24.08 ± 3.60ng/dl in CGP with DM. All three groups found a statistically significant difference in mean Apelin Level, PPD, and CAL.

Conclusion: This study indicated a possible role of adipokines in inflammation and glucose level regulation in patients with T2DM and CGP. In the coming era, apelin levels in saliva can be used for screening objectives in large populations to assure the risk of destructive periodontal disease.

Bangladesh Journal of Medical Science Vol. 22 No. 02 April’23 Page : 305-315

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Published

2023-04-11

How to Cite

Hirani, T. ., Haq, M. A. ., Shetty, N. J. ., Patel, V. ., Ahmad, R. ., Sinha, S. ., Dutta, S. ., Haque, M. ., & Kumar, S. . (2023). Evaluating Salivary Apelin Level among Chronic Periodontitis Patients, with and without Type 2 Diabetes Mellitus utilizing Enzyme‑Linked Immunosorbent Assay: A Comparative Study. Bangladesh Journal of Medical Science, 22(2), 305–315. https://doi.org/10.3329/bjms.v22i2.64987

Issue

Vol. 22 No. 2 (2023)

Section

Original Articles

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Copyright (c) 2023 Tanvi Hirani, Md Ahsanul Haq, Neetha J Shetty, Viral Patel, Rahnuma Ahmad, Susmita Sinha, Siddhartha Dutta, Mainul Haque, Santosh Kumar

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