All-Trans Retinoic Acid Reduces Matrix Metalloproteinase-2 and Increases E-Cadherin Levels in BeWo Choriocarcinoma Cells

Authors

  • Tatit Nurseta Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University – RSUD Dr. Saiful Anwar, Malang, East Java, Indonesia
  • Stefani Harumsari Resident of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
  • Nanda Agus Prasetya Resident of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
  • I Wayan Agung Indrawan Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University – RSUD Dr. Saiful Anwar, Malang, East Java, Indonesia

DOI:

https://doi.org/10.3329/bjms.v22i2.64992

Keywords:

ATRA, BeWo cell line; choriocarcinoma; MMP-2; E-cadherin

Abstract

Background: Choriocarcinoma is a malignant trophoblastic tumor that can degrade the uterine basement membrane to facilitate local and distant metastasis. Matrix metalloproteinase-2 (MMP-2) is a vital proteinase produced by trophoblasts and can cleave type IV collagen in the basement membrane of the uterine epithelium. In addition, E-cadherin controls cell adhesion, which is associated with tumorprogression, and loss of E-cadherin function is associated with metastasis. Vitamin A analogues, including all-trans retinoic acid (ATRA), exhibit anticancer properties, including metastasis inhibition.This study aimed to investigate the ability of ATRA to prevent trophoblast invasion and metastasis in choriocarcinoma by assessing MMP-2 and E-cadherin activity.

Methods: This study administered various doses of ATRA to the BeWo (American Type Culture Collection CCL-98) choriocarcinoma cell line and assessed MMP-2 and E-cadherin activity through flow cytometry. The research was performed at the Biomedical Laboratory of the Faculty of Medicine of Universitas Brawijaya Malang, East Java, Indonesia.

Results: BeWo cells were exposed to 0, 50, 100, 200, 400, or 800 μg/mL ATRA. MMP-2 was downregulated after ATRA treatment. The greatest reduction in MMP-2 level was observed after 200 μg/mL ATRA treatment, but this result was not statistically significant (P = 0.550). However, ATRA treatment significantly reduced the number of BeWo cells with low E-cadherin levels (P = 0.012).

Conclusion: ATRA downregulates MMP-2 and upregulates E-cadherin. Further in vitro and animal studies are required to evaluate the ability of ATRA to inhibit metastasis.

Bangladesh Journal of Medical Science Vol. 22 No. 02 April’23 Page : 336-340

Downloads

Download data is not yet available.
Abstract
39
PDF
89

Downloads

Published

2023-04-11

How to Cite

Nurseta, T. ., Harumsari, S. ., Prasetya, N. A. ., & Indrawan, I. W. A. . (2023). All-Trans Retinoic Acid Reduces Matrix Metalloproteinase-2 and Increases E-Cadherin Levels in BeWo Choriocarcinoma Cells. Bangladesh Journal of Medical Science, 22(2), 336–340. https://doi.org/10.3329/bjms.v22i2.64992

Issue

Vol. 22 No. 2 (2023)

Section

Original Articles

License

Copyright (c) 2023 Tatit Nurseta, Stefani Harumsari, Nanda Agus Prasetya, I Wayan Agung Indrawan

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.