In vitro cytogenetic effects of Hypericum heterophyllum in human peripheral blood lymphocytes
DOI:
https://doi.org/10.3329/bjp.v7i1.10119Keywords:
Hypericum heterophyllum, Cytogenetic, Mitotic index, Replication indexAbstract
Hypericum species have been used in traditional medicine in Turkey for thousands of years. In order to determine the cytogenetic effects of Hypericum heterophyllum, human lymphocytes were incubated with the aqueous extracts. Mitotic index frequencies and replication index values were increased with increasing extract concentrations of H. heterophyllum. The results indicate the cytotoxic effects as well as proliferative effects and suggest that the extracts of the compounds exhibit cytotoxic properties as well as mitotic and proliferative properties.
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Crockett SL, Robson NKB. Taxonomy and chemotaxonomy of the Genus Hypericum. In: Hypericum. Odabas MS, Ç?rak C, eds. Medicinal and Aromatic Plant Science and Biotechnology 5, 2011, pp 1-13.
Cakir A, Kordali S, Zengin H, Izumi S, Hirata T. Composition and antifungal activity of Hypericum hyssopifolium and Hypericum heterophyllum. Flavour Frag. 2004; 19: 62-68.
Ero?lu HE. Determination of genotoxic effects of Turkey Helichrysum Mill. (Asteraceae) taxa. PhD Thesis. Erciyes University, Kayseri, Turkey, 2008.
Fenech M, Bonassi S. The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes. Mutagenesis. 2011; 26: 43-49.
Holland N, Duramad P, Rothman N, Figgs LW, Blair A, Hubbard A, Smith MT. Micronucleus frequency and proliferation in human lymphocytes after exposure to herbicide 2,4-dichlorophenoxyacetic acid in vitro and in vivo. Mutat Res. 2002; 521: 165-78.
Norppa H, Falck GC. What do human micronuclei contain? Mutagenesis 2003; 18: 221-33.
Pastor S, Gutiérrez S, Creus A, Cebulska-Wasilewska A, Marcos R. Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides. Mutat Res. 2001; 495: 147-56.
Shin IS, Seo CS, Ha HK, Lee MY, Huang DS, Huh JI, Shin HK. Genotoxicity assessment of Pyungwisan (PWS), a traditional herbal prescription. J Ethnopharmaco. 2011; 133: 696-703.
Tucker JD, Nath J, Hando JC. Activation status of the X chromosome in human micronucleated lymphocytes. Hum Genet. 1996; 97: 471-75.
Walker PMB. The mitotic index and interphase processes. J Exp Biol. 1952; 31: 8-15.
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