Phytochemical composition, mosquito larvicidal, ovicidal and repellent activity of Calotropis procera against Culex tritaeniorhynchus and Culex gelidus

Authors

  • Gaurav Kumar Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore
  • Loganathan Karthik Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore
  • Bhaskara Rao Kokati Venkata Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore
  • Arivarasan Vishnu Kirthi Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore, Tamil Nadu
  • Chidambaram Jayaseelan Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore, Tamil Nadu
  • Abdul Abdul Rahuman Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore, Tamil Nadu

DOI:

https://doi.org/10.3329/bjp.v7i1.10156

Keywords:

Calotropis procera, larvicidal activity, ovicidal activity, repellent activity, Culex tritaeniorhynchus, Culex gelidus

Abstract

Focus of this study was to determine the phytochemical composition and mosquito controlling potential of aqueous extract of Calotropis procera (Ait.) R.Br. leaves using in vitro methods. Preliminary phytochemical analysis of the extract showed the presence of phenolic compounds, flavonoids, alkaloids, tannins, saponins, glycosides and phytosterols as major phytochemical groups. Aqueous extract of C. procera leaves (1,000 ppm) exhibited 100% larvicidal activity against fourth instar larvae of Culex tritaeniorhynchus and Cx. gelidus. Extract treatment (1,000 ppm) of both mosquitoes eggs resulted in to 100% ovicidal activity. At 1,000 ppm, extract provided complete protection from mosquito bite for 240 min against both mosquitoes; however at lower doses the protection time was less. The findings of the current study emphasise the potentiality of C. procera leaves for controlling the mosquito population and their possible way in the developing the natural insecticide for the control of Cx. tritaeniorhynchus and Cx. gelidus mosquitoes.

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References

Al-Rajhy D, Hussein HI, El-Osta MS, Ali AG. Larvicidal and ovipositional activity of Calotropis procera, Neemazal/T and Eucalyptus spp. against Culex pipiens. J Pest Cont Environ Sci. 2000; 8: 15-26.

Bhaskar VH, Ajay SS. Antimicrobial activity of Calotropis procera seeds. Asian J Chem. 2009; 21: 5788-90.

CDC. Japanese encephalitis vaccines: Recommendations of the Advisory Committee on Immunization Practices (ACIP).MMWR 2010; 59 (No. RR-1), 2010, pp 1-27.

Chenniappan K, Kadarkarai M. Oviposition deterrent, ovicidal and gravid mortality effects of ethanolic extract of Andrographis paniculata Nees against the malarial vector Anopheles stephensi Liston (Diptera: Culicidae). Entomol Res. 2008; 38: 119-25.

Das D, Chandra G. Mosquito larvicidal activity of Rauvolfia serpentina L. seeds against Culex quinquefasciatus Say. Asian Pac J Trop Med. 2012; 5: 42-45.

Dewan S, Sangraula H, Kumar VL. Preliminary studies on the analgesic activity of latex of Calotropis procera. J Ethnopharmacol. 2000; 73: 307-11.

Elimam AM, Elmalik KH, Ali FS. Efficacy of leaves extract of Calotropis procera Ait. (Asclepiadaceae) in controlling Anopheles arabiensis and Culex quinquefasciatus mosquitoes. Saudi J Biol Sci. 2009; 16: 95-100.

Eskenazi B, Rosas LG, Marks AR, Bradman A, Harley K, Holland N, Johnson C, Barr DB. Pesticide toxicity and the developing brain. Basic Clin Pharmacol Toxicol. 2008; 102: 228-36.

Fradin MS, Day JF. Comparative efficacy of insect repellents against mosquito bite. New Engl J Med. 2002; 3471: 13-18.

Govindarajan M, Jebanesan A, Pushpanathan T. Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitol Res. 2008; 102: 289-92.

Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A. Mosquito larvicidal, ovicidal, and repellent properties of botanical extracts against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res. 2011; 109: 353-67.

Harborne JB. Phytochemical methods. A guide to modern techniques of plant analysis. 3rd ed. Chapman and Hall, London, 1998, pp 40-96.Isman MB. Botanical insecticides: for richer, for poorer. Pest Manag Sci. 2008; 64: 8-11.

Jaga K, Brosius D. Pesticide exposure: human cancers on the horizon. Rev Environ Health. 1999; 14: 39-50.

Jalalpure SS, Salahuddin M, Imtiyaz Shaikh M, Manvi FV. Anticonvulsant effects of Calotropis procera root in rats. Pharma Biol. 2009; 47: 162-7.

Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G. Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res. 2008; 104: 1163-71.

Kamath JV, Rana AC. Preliminary study on antifertility activity of Calotropis procera roots in female rats. Fitoterapia 2002; 73: 111-15.

Kumar K, Sharma AK, Kumar S, Patel S, Sarkar M, Chauhan LS. Multiple insecticide resistance/susceptibility status of Culex quinquefasciatus, principal vector of bancroftian filariasis from filaria endemic areas of northern India. Asian Pac J Trop Med. 2011; 4: 426-29.

Kumar S, Dewan S, Sangraula H, Kumar VL. Anti-diarrhoeal activity of the latex of Calotropis procera. J Ethnopharmacol. 2001; 76: 115-18.

Kuta FA. Antifungal effect of Calotropis procera stem bark on Epidermophyton flocosum and Trichophyton gypseum. Afr J Biotechnol. 2008; 7: 2116-18.

Maia MF, Moore SJ. Plant-based insect repellents: a review of their efficacy, development and testing. Malar J. 2011; 10: S11.

Mathur R, Gupta SK, Mathur SR, Velpandian T. Anti-tumor studies with extracts of Calotropis procera (Ait.) R.Br. root employing Hep2 cells and their possible mechanism of action. Indian J Exp Biol. 2009; 47: 343-48.

Moustafa AMY, Ahmed SH, Nabil ZI, Hussein AA, Omran MA. Extraction and phytochemical investigation of Calotropis procera: Effect of plant extracts on the activity of diverse muscles. Pharm Biol. 2010; 48: 1080-90.

Murti Y, Yogi B, Pathak D. Pharmacognostic standardization of leaves of Calotropis procera )Ait.) R. Br. (Asclepiadaceae). Int J Ayurveda Res. 2010; 1: 14-17.

Raaman N. Phytochemical techniques. 1st ed. New India Publication Agency, New Delhi, 2006, pp 19-24.

Raghavendra K, Verma V, Srivastava HC, Gunasekaran K, Sreehari U, Dash AP. Persistence of DDT, malathion & deltamethrin resistance in Anopheles culicifacies after their sequential withdrawal from indoor residual spraying in Surat district, India. Indian J Med Res. 2010; 132: 260-64.

Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B. Effect of Feronia limonia on mosquito larvae. Fitoterapia 2000; 71: 553-55.

Rahuman AA, Bagavan A, Kamaraj C, Saravanan E, Zahir AA, Elango G. Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res. 2009; 104: 1365-72.

Rajkumar S, Jebanesan A. Larvicidal and oviposition activity of Cassia obtusifolia Linn (Family: Leguminosae) leaf extract against malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res. 2009; 104: 337-40.

Ramachandra Setty S, Quereshi AA, Viswanath Swamy AH, Patil T, Prakash T, Prabhu K et al. Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia 2007; 78: 451-54.

Ramos MV, Glaís de Paiva B, Teixeira de Freitas CD, Nogueira NAP, Alencar NMN, Simão de Sousa PA, Carvalho AFU. Latex constituents from Calotropis procera (R. Br.) display toxicity upon egg hatching and larvae of Aedes aegypti (Linn.). Mem Inst Oswaldo Cruz, Rio de Janeiro. 2006; 101:503-10.

Reddy PJ, Krishna D, Murthy US, Jamil K. A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. CABIOS.1992; 8: 209-13.

Roy S, Sehgal R, Padhy BM, Kumar VL. Anti-oxidant and protective effect of latex of Calotropis procera against alloxan- induced diabetes in rats. J Ethnopharmacol. 2005; 102: 470-73.

Self LS, Shin HK, Kim KH, Lee KW, Chow CY, Hong HK. Ecological studies on Culex tritaeniorhynchus as a vector of Japanese encephalitis. Bull World Health Organ. 1973; 49: 41-47.

Shahi M, Hanafi-Bojd AA, Iranshahi M, Vatandoost H, Hanafi- Bojd MY. Larvicidal efficacy of latex and extract of Calotropis procera (Gentianales: Asclepiadaceae) against Culex quinquefasciatus and Anopheles stephensi (Diptera: Culicidae). J Vector Borne Dis. 2010; 47: 185-88.

Sharma P, Sharma JD. In vitroSchizonticidal screening of Calotropis procera. Fitoterapia 2000; 71: 77-79.

Sharma VP. Health hazards of mosquito repellents and safe alternatives. Curr Sci. 2001; 80: 341-43.

Singh H, Kumar S, Dewan S, Kumar VL. Inflammation induced by latex of Calotropis procera: A new model to evaluate anti-inflammatory drugs. J Pharmacol Toxicol Methods. 2000; 43: 219-24.

Singh RK, Mittal PK, Dhiman RC. Laboratory study on larvicidal properties of leaf extract of Calotropis procera (Family-Asclepiadaceae) against mosquito larvae. J Commun Dis. 2005; 37: 109-13.

Soares PM, Lima SR, Matos SG, Andrade MM, Patrocinio MCA, De Freitas CDT, Ramos MV, Vasconcelos SMM. Antinociceptive activity of Calotropis procera latex in mice. J Ethnopharmacol. 2005; 99: 125-29.

Su T, Mulla MS. Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Am Mosq Control Assoc. 1998; 14: 204-09.

Venkatachalam MR, Jebanesan A. Repellent activity of Ferronia elephantum Corr. (Rutaceae) leaf extract against Aedes aegypti (L.). Bioresour Technol. 2001; 76: 287-88.

WHO. Report of the WHO informal consultation on the evaluation on the testing of insecticides, CTD/WHO PES/ IC/96.1, 1996 ,pp 30-40.

Zafar I, Muhammad L, Jabbar A. Anthelmintic activity of Calotropis procera (Ait.) Ait. F. flowers in sheep. J Ethnopharmacol. 2005; 102: 256-61.

Additional Files

Published

2012-05-28

How to Cite

Kumar, G., L. Karthik, B. R. Kokati Venkata, A. V. Kirthi, C. Jayaseelan, and A. A. Rahuman. “Phytochemical Composition, Mosquito Larvicidal, Ovicidal and Repellent Activity of Calotropis Procera Against Culex Tritaeniorhynchus and Culex Gelidus”. Bangladesh Journal of Pharmacology, vol. 7, no. 1, May 2012, pp. 63-69, doi:10.3329/bjp.v7i1.10156.

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Research Articles