Antiamoebic activity of marine sponge Haliclona exigua (Krikpatrick)
DOI:
https://doi.org/10.3329/bjp.v4i1.1083Keywords:
Antiamoebic activity, Araguspongin C, Haliclona exigua, Marine spongeAbstract
The methanol and methanol-chloroform (1:1) extracts of the freshly collected Haliclona exigua showed minumim inhibitory concentration (MIC) of 125 ?g/mL and 250 ?g/mL respectively in in vitro studies, but when both of these were tested in vivo in rats, only methanol-chloroform showed 80% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entameba histolytica. Therefore only methanol-chloroform extract was further fractionated into four fractions (n-hexane, chloroform, n-butanol soluble and n-butanol insoluble fractions). Out of these, only n-hexane and n-butanol soluble fractions showed 80% inhibition of trophozoites at 900 mg/kg dose. Further the chromatography of the n-butanol fraction yielded araguspongin-C which showed promising results at different doses.
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References
Beltran F. Epidemiologica de las infeciones on Entameba histolytica. Sect. VIII, Protozoan diseases. Proceedings of the 4th International Congress on Tropical Medicine and Malaria, Washington, 1948 p 1056.
Bhosale SH, Jagtap TG, Naik CG. Antifungal activity of some marine organisms from India against food spaelage Aspergillus strains. Mycopathologia 1999; 147: 133-38.
Compagnone RS, Pina IC, Rangel HR, Dagger F, Suarez AI, Reddy MVR, Faulkner DJ. Antileishmanial cyclic peroxides from the palauan sponge Plakortis aff.angulospiculatus. Tetrahedron 1998; 54: 3057-68.
Copp BR, Kayser O, Brun R, Kiderlen AF. Antiparasitic activity of marine pyridoacridone alkaloids related to the ascididemnins. Planta Med. 2003; 69: 527-31.
Das SR. A novel and rapid method for in vitro testing of antiamebic agents against aerobic and anaerobic amoebae growing axenically or with bacteria. Curr Sci. 1975; 44: 463-64.
Diamond LS, Harlow DR, Cunnick CC. A new medium for the axenic cultivation of Entameba histolytica and other Entameba. Trans Roy Soc Trop Med Hyg. 1978; 72: 431-32.
Edrada RA, Proksh P, Wray V, Wilte L, Ofrogen LV. Four new bioactive lobane diterpenes of the soft coral Lobophytum paucifolrum from Mindoro, Philippines. J Nat Prod. 1998; 61: 358-61.
Faulkner DJ. Marine natural products. Nat Prod Rep. 2001; 18: 1-49.
Haefner B. Drugs from the deep: Marine natural products as drug candidates. Drug Discov Today. 2003; 8: 536-44.
Leitch GJ. Intestinal luminal and mucosal microclimate H- and NH+ concentration as factors in the etiology of experimental amebiasis. Am J Trop Med Hyg. 1988; 38: 480-96.
Le Pape P, Zidane M, Abdala H, More M. A glycoprotein isolated from the sponge, Pachymatisma johnstonii, has anti-leishmanial activity. Cell Biol Int. 2000; 24: 5156.
Marchan E, Arrieche D, Henriquez W, Crescente O. In vitro effect of an alkaloid isolated from Amphimedon viridis (Porifea) on promastigotes of Leishmania mexicana. Rev Biol Trop. 2000; 48 (Suppl): 31-38.
Nakao Y, Shiroiwa T, Murayama S, Matsunaga S, Goto Y, Matsumoto Y, Fusetani N. Identification of renieramycin A as an antileishmanial substance in a marine sponge Neopetrosia sp. Marine Drugs. 2004; 2: 55-62.
Neal RA. Some observations in the variations of virulence and response to chemotherapy of strains of Entameba histolytica. Trans Roy Soc Trop Med Hyg. 1951; 44: 439-52.
Prasad BN, Bansal I. Interrelationship between fecal pH and susceptibility to Entameba histolytica infection of rats. Trans Roy Soc Trop Med Hyg. 1983; 77: 271-74.
Rangel HR, Dagger F. Antiproliferative effect of illimaquinone on Leishmania Mexicana. Cell Biol Int. 1997; 21: 337-39.
Rao KV, Santarsiero BD, Mesecar AD, Schinazi RF, Tekwani BL, Hamann MT. New manzamine alkaloids with activity against infectious and tropical parasitic diseases from an Indonesian sponge. J Nat Prod. 2003; 66: 823-28.
Rao KV, Kasanah N, Wahyuono S, Tekwani BL, Schinazi RF, Hamann MT. Three new manzamine alkaloids from a common Indonesian sponge and their activity against infectious and tropical parasitic diseases. J Nat Prod. 2004; 67: 1314-18.
Reddy MVR, Faulkner DJ. 3?, 3?-Dimethylxestospongin-C, a new bis-1-oxaquinolizidinealkaloid from the Palam sponge Xestospongia species. Nat Prod Lett. 1997; 11: 53-59.
Robinson GR. Laboratory cultivation of some human parasitic amoeba. J Gen Microbiol. 1968; 53: 19-29.
Stanley SL Jr. Amebiasis. Lancet 2003; 361: 102534.
Shin J, Fenical W. Fuscosides A-D anti-inflammatory diterpenoid glycoside of new structural classes from the Caribbean gorgonian Eunicea fusca. J Org Chem. 1991; 56: 3153-58.
Venkateshwara RJ. Desaih D. Vig PJ, Venkateshwarlu Y. Marine biomolecules inhibit rat brain nitric oxide synthase activity. Toxicology 1998; 129: 103-12.
Venkateshwarlu Y, Reddy MVR. Rao JV. Bis-1-oxaquinolizidines from the sponge Haliclona exigua. J Nat Prod. 1994; 57: 1283-85.
Yan HY. Harvesting drugs from the seas and how Taiwan could contribute to this effort. Changhua J Med. 2004; 9: 1-6.
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