Antiamoebic activity of marine sponge Haliclona exigua (Krikpatrick)

Authors

  • V. Lakshmi Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow 226001
  • A. Saxena Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow 226001
  • S. K. Mishra Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow 226001
  • M. Mishra Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow 226001
  • S. Srivastava Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow 226001, India.
  • S. Ghoshal Divisions of Microbiology, Central Drug Research Institute, Lucknow 226001

DOI:

https://doi.org/10.3329/bjp.v4i1.1083

Keywords:

Antiamoebic activity, Araguspongin C, Haliclona exigua, Marine sponge

Abstract

The methanol and methanol-chloroform (1:1) extracts of the freshly collected Haliclona exigua showed minumim inhibitory concentration (MIC) of 125 ?g/mL and 250 ?g/mL respectively in in vitro studies, but when both of these were tested in vivo in rats, only methanol-chloroform showed 80% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entameba histolytica. Therefore only methanol-chloroform extract was further fractionated into four fractions (n-hexane, chloroform, n-butanol soluble and n-butanol insoluble fractions). Out of these, only n-hexane and n-butanol soluble fractions showed 80% inhibition of trophozoites at 900 mg/kg dose. Further the chromatography of the n-butanol fraction yielded araguspongin-C which showed promising results at different doses.

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Additional Files

Published

2008-09-22

How to Cite

Lakshmi, V., A. Saxena, S. K. Mishra, M. Mishra, S. Srivastava, and S. Ghoshal. “Antiamoebic Activity of Marine Sponge Haliclona Exigua (Krikpatrick)”. Bangladesh Journal of Pharmacology, vol. 4, no. 1, Sept. 2008, pp. 55-59, doi:10.3329/bjp.v4i1.1083.

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Research Articles