Evaluation of antimicrobial activities of extracts of endophytic fungi from Artemisia annua
DOI:
https://doi.org/10.3329/bjp.v7i2.10951Keywords:
Antimicrobial, Artemisia annua, Endophytic fungusAbstract
The endophytic extracts of 11 fungi associated with asympomatic Artemisia annua Linn., were evaluated for antimicrobial activity against three human pathogenic microbes, Escherichia coli, Staphylococcus aureus and Trichophyton rubrum, and two plant pathogens, Rhizoctonia cerealis and Magnaporthe grisea. The results showed that these endophytic extracts had different inhibitory effects on microbial pathogens at 100 mg/mL. Among these fungal endophytes, three strains Aspergillus spp. SPS-02, SPS-04 and SPS-01 respectively showed the strongest antimicrobial activities against E. coli, S. aureu, T. rubrum. An endophytic Mucor sp. SPS-11 had the most pronounced effect on R. cerealis. Two strains Aspergillus sp. SPS-02 and Cephalosporium sp. SPS-08 exhibited the strongest antimicrobial activities against M. grisea. These anti-pathogenic endophytes could be applied as new sources of antibiotics in agriculture and pharmaceutical industry.
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Bdha BS. Molecular and functional characterization of endophytic fungi from traditional medicinal plants. World J Microbiol Biotechnol. 2012; 28: 96371.
Deng ZJ, Cao LX, Huang HW, Jiang XY, Wang WF, Shi Y, Zhang RD. Characterization of Cd- and Pb-resistant fungal endophyte Mucor sp. CBRF59 isolated from rapes (Brassica chinensis) in a metal-contaminated soil. J Hazard Mater. 2011; 185: 71724.
Ding L, Dahse HM, Hertweck C. Cytotoxic alkaloids from Fusarium incarnatum associated with the mangrove tree Aegiceras corniculatum. J Nat Prod. 2012; 75: 61721.
Li Y, Niu SB, Sun BD, Liu SC, Liu XZ, Che YS. Cytosporolides A-C, antimicrobial meroterpenoids with a unique peroxylactone skeleton from Cytospora sp. Org Lett. 2010; 12: 314447.
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Rouveix B. Antibiotic safety assessment. Int J Antimicrob Agents. 2003; 21: 21521.
Simoes M, Bennett RN, Rosa EAS. Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Nat Prod Rep. 2009; 26: 74657.
Sun HF, Li XM, Meng L, Cui CM, Gao SS, Li CS, Huang CG, Wang BG. Asperolides A-C, tetranorlabdane diterpenoids from the marine alga-derived endophytic fungus Aspergillus wentii EN-48. J Nat Prod. 2012; 75: 14852.
Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep. 2001; 18: 44859.
Wei JC. Handbook of Fungus Identification. Shanghai, China, Shanghai Science & Technology Press, 1979 (in Chinese).
Xue H, Lu CH, Liang LY, Shen YM. Secondary metabolites of Aspergillus sp. CM9a, an endophytic fungus of Cephalotaxus mannii. Rec Nat Prod. 2012; 6: 2834.
Zaika LL. Spices and herbs: Their antimicrobial activity and its determination. J Food Safety. 1998; 9: 97118.
Zhan JX, Gunaherath GMKB, Wijeratne EMK, Gunatilaka AAL. Asperpyrone D and other metabolites of the plant-associated fungal strain Aspergillus tubingensis. Phytochemistry 2007; 68: 36872.
Zhang HW, Huang WY, Song YC, Chen JR, Tan RX. Four 6H-dibenzo[b,d]pyran-6-one derivatives produced by the endophyte Cephalosporium acremonium IFB-E007. Helv Chim Acta. 2005; 88: 286164.
Zhang HW, Song YC, Tan RX. Biology and chemistry of endophytes. Nat Prod Rep. 2006; 23: 75371.
Zhang HW, Huang WY, Chen JR, Yan WZ, Xie DQ, Tan RX. Cephalosol: an antimicrobial metabolite with an unprecedented skeleton from endophytic Cephalosporium acremonium IFB-E007. Chem Eur J. 2008; 14: 1067074.
Zhang HW, Zhang J, Hu S, Zhang ZJ, Zhu CJ, Tan RX. Ardeemins and cytochalasins from Aspergillus terrus residing in Artemisia annua. Planta Med. 2010; 76: 161621.
Zhao K, Ping W, Li Q, Hao S, Zhao L, Gao T, Zhou D. Aspergillus niger var. taxi, a new species variant of taxol-producing fungus isolated from Taxus cuspidata in China. J Appl Microbiol. 2009; 107: 120207.
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