Evaluation of antimicrobial activities of extracts of endophytic fungi from Artemisia annua
DOI:
https://doi.org/10.3329/bjp.v7i2.10951Keywords:
Antimicrobial, Artemisia annua, Endophytic fungusAbstract
The endophytic extracts of 11 fungi associated with asympomatic Artemisia annua Linn., were evaluated for antimicrobial activity against three human pathogenic microbes, Escherichia coli, Staphylococcus aureus and Trichophyton rubrum, and two plant pathogens, Rhizoctonia cerealis and Magnaporthe grisea. The results showed that these endophytic extracts had different inhibitory effects on microbial pathogens at 100 mg/mL. Among these fungal endophytes, three strains Aspergillus spp. SPS-02, SPS-04 and SPS-01 respectively showed the strongest antimicrobial activities against E. coli, S. aureu, T. rubrum. An endophytic Mucor sp. SPS-11 had the most pronounced effect on R. cerealis. Two strains Aspergillus sp. SPS-02 and Cephalosporium sp. SPS-08 exhibited the strongest antimicrobial activities against M. grisea. These anti-pathogenic endophytes could be applied as new sources of antibiotics in agriculture and pharmaceutical industry.
Downloads
629
187 Read
8
References
Bhagat J, Kaur A, Sharma M, Saxena AK, Chadha BS. Molecular and functional characterization of endophytic fungi from traditional medicinal plants. World J Microbiol Biotechnol. 2012; 28: 96371.
Deng ZJ, Cao LX, Huang HW, Jiang XY, Wang WF, Shi Y, Zhang RD. Characterization of Cd- and Pb-resistant fungal endophyte Mucor sp. CBRF59 isolated from rapes (Brassica chinensis) in a metal-contaminated soil. J Hazard Mater. 2011; 185: 71724.
Ding L, Dahse HM, Hertweck C. Cytotoxic alkaloids from Fusarium incarnatum associated with the mangrove tree Aegiceras corniculatum. J Nat Prod. 2012; 75: 61721.
Li Y, Niu SB, Sun BD, Liu SC, Liu XZ, Che YS. Cytosporolides A-C, antimicrobial meroterpenoids with a unique peroxylactone skeleton from Cytospora sp. Org Lett. 2010; 12: 314447.
Li XJ, Zhang Q, Zhang AL, Gao JM. Metabolites from Aspergillus fumigatus, an endophytic fungus associated with Melia azedarach, and their antifungal, antifeedant, and toxic activities. J Agric Food Chem. 2012; 60: 342431.
Rouveix B. Antibiotic safety assessment. Int J Antimicrob Agents. 2003; 21: 21521.
Simoes M, Bennett RN, Rosa EAS. Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Nat Prod Rep. 2009; 26: 74657.
Sun HF, Li XM, Meng L, Cui CM, Gao SS, Li CS, Huang CG, Wang BG. Asperolides A-C, tetranorlabdane diterpenoids from the marine alga-derived endophytic fungus Aspergillus wentii EN-48. J Nat Prod. 2012; 75: 14852.
Tan RX, Zou WX. Endophytes: A rich source of functional metabolites. Nat Prod Rep. 2001; 18: 44859.
Wei JC. Handbook of fungus identification. Shanghai, China. Shanghai Science & Technology Press, 1979 (in Chinese).
Xue H, Lu CH, Liang LY, Shen YM. Secondary metabolites of Aspergillus sp. CM9a, an endophytic fungus of Cephalotaxus mannii. Rec Nat Prod. 2012; 6: 2834.
Zaika LL. Spices and herbs: their antimicrobial activity and its determination. J Food Safety. 1998; 9: 97118.
Zhan JX, Gunaherath GMKB, Wijeratne EMK, Gunatilaka AAL. Asperpyrone D and other metabolites of the plant-associated fungal strain Aspergillus tubingensis. Phytochemistry 2007; 68: 36872.
Zhang HW, Huang WY, Song YC, Chen JR, Tan RX. Four 6H-dibenzo[b,d]pyran-6-one derivatives produced by the endophyte Cephalosporium acremonium IFB-E007. Helv Chim Acta. 2005; 88: 286164.
Zhang HW, Song YC, Tan RX. Biology and chemistry of endophytes. Nat Prod Rep. 2006; 23: 75371.
Zhang HW, Huang WY, Chen JR, Yan WZ, Xie DQ, Tan RX. Cephalosol: An antimicrobial metabolite with an unprecedented skeleton from endophytic Cephalosporium acremonium IFB-E007. Chem Eur J. 2008; 14: 1067074.
Zhang HW, Zhang J, Hu S, Zhang ZJ, Zhu CJ, Tan RX. Ardeemins and cytochalasins from Aspergillus terrus residing in Artemisia annua. Planta Med. 2010; 76: 161621.
Zhao K, Ping W, Li Q, Hao S, Zhao L, Gao T, Zhou D. Aspergillus niger var. taxi, a new species variant of taxol-producing fungus isolated from Taxus cuspidata in China. J Appl Microbiol. 2009; 107: 120207.
Bdha BS. Molecular and functional characterization of endophytic fungi from traditional medicinal plants. World J Microbiol Biotechnol. 2012; 28: 96371.
Deng ZJ, Cao LX, Huang HW, Jiang XY, Wang WF, Shi Y, Zhang RD. Characterization of Cd- and Pb-resistant fungal endophyte Mucor sp. CBRF59 isolated from rapes (Brassica chinensis) in a metal-contaminated soil. J Hazard Mater. 2011; 185: 71724.
Ding L, Dahse HM, Hertweck C. Cytotoxic alkaloids from Fusarium incarnatum associated with the mangrove tree Aegiceras corniculatum. J Nat Prod. 2012; 75: 61721.
Li Y, Niu SB, Sun BD, Liu SC, Liu XZ, Che YS. Cytosporolides A-C, antimicrobial meroterpenoids with a unique peroxylactone skeleton from Cytospora sp. Org Lett. 2010; 12: 314447.
Li XJ, Zhang Q, Zhang AL, Gao JM. Metabolites from Aspergillus fumigatus, an endophytic fungus associated with Melia azedarach, and their antifungal, antifeedant, and toxic activities. J Agr Food Chem. 2012; 60: 342431.
Rouveix B. Antibiotic safety assessment. Int J Antimicrob Agents. 2003; 21: 21521.
Simoes M, Bennett RN, Rosa EAS. Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Nat Prod Rep. 2009; 26: 74657.
Sun HF, Li XM, Meng L, Cui CM, Gao SS, Li CS, Huang CG, Wang BG. Asperolides A-C, tetranorlabdane diterpenoids from the marine alga-derived endophytic fungus Aspergillus wentii EN-48. J Nat Prod. 2012; 75: 14852.
Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep. 2001; 18: 44859.
Wei JC. Handbook of Fungus Identification. Shanghai, China, Shanghai Science & Technology Press, 1979 (in Chinese).
Xue H, Lu CH, Liang LY, Shen YM. Secondary metabolites of Aspergillus sp. CM9a, an endophytic fungus of Cephalotaxus mannii. Rec Nat Prod. 2012; 6: 2834.
Zaika LL. Spices and herbs: Their antimicrobial activity and its determination. J Food Safety. 1998; 9: 97118.
Zhan JX, Gunaherath GMKB, Wijeratne EMK, Gunatilaka AAL. Asperpyrone D and other metabolites of the plant-associated fungal strain Aspergillus tubingensis. Phytochemistry 2007; 68: 36872.
Zhang HW, Huang WY, Song YC, Chen JR, Tan RX. Four 6H-dibenzo[b,d]pyran-6-one derivatives produced by the endophyte Cephalosporium acremonium IFB-E007. Helv Chim Acta. 2005; 88: 286164.
Zhang HW, Song YC, Tan RX. Biology and chemistry of endophytes. Nat Prod Rep. 2006; 23: 75371.
Zhang HW, Huang WY, Chen JR, Yan WZ, Xie DQ, Tan RX. Cephalosol: an antimicrobial metabolite with an unprecedented skeleton from endophytic Cephalosporium acremonium IFB-E007. Chem Eur J. 2008; 14: 1067074.
Zhang HW, Zhang J, Hu S, Zhang ZJ, Zhu CJ, Tan RX. Ardeemins and cytochalasins from Aspergillus terrus residing in Artemisia annua. Planta Med. 2010; 76: 161621.
Zhao K, Ping W, Li Q, Hao S, Zhao L, Gao T, Zhou D. Aspergillus niger var. taxi, a new species variant of taxol-producing fungus isolated from Taxus cuspidata in China. J Appl Microbiol. 2009; 107: 120207.
Additional Files
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
