Anti-candida activity by Hymenocallis littoralis extracts for opportunistic oral and genital infection Candida albicans

Authors

  • Jeevandran Sundarasekar School of Biological Sciences, Universiti Sains Malaysia
  • Geethaa Sahgal Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia
  • Sreeramanan Subramaniam School of Biological Sciences, Universiti Sains Malaysia

DOI:

https://doi.org/10.3329/bjp.v7i3.11625

Keywords:

Candida albicans, Hymenocallis littoralis, TEM, SEM

Abstract

Candida albicans is a unicellular fungus that causes an opportunistic infection in the oral cavity and vagina. The present article reports the inhibitory effects of Hymenocallis littoralis methanol sonication extracts against Candida albicans. This plant has been used widely in traditional medicine and has proven to possess anti-cancer properties for numerous cell lines. Various plant parts such as bulb, flower, anther, root, leaves and stem were tested against this opportunistic organism and found that the flower and anther were effective at 6.25 mg/mL. This is the first report on anti-candida activity of H. littoralis methanol extract. Transmission (TEM) and scanning electron microscope (SEM) observations were used to observe the cytological and morphological expression of the extract treated C. albicans. TEM reveal the complete destruction of nucleus and internal organelles in the yeast like fungus cell. SEM reveals the fissure on the membrane cells. The methanol crude extract has anti-candida activity for tested concentration.

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References

Abou-Donia AH, Toaima SM, Hammoda HM, Shawky E, Kinoshita E, Takayama H. Phytochemical and Biological Investigation of Hymenocallis littoralis Salisb. Chem Biodivers. 2008; 5: 332-40.

Backhaus RA, Pettit III GR, Huang DS, Pettit GR, Groszek G, Odgers JC, Ho J, Meerow A. Biosynthesis of the antineoplastic pancratistatin following tissue culture of Hymenocallis littoralis (Amaryllidaceae). Acta Hortic. 1992; 306: 364-66.

Canilac N, Mourey A. Anti-bacterial activity of the essential oil of Picea excels on Listeria, Staphylococcus aureus and coliform bacteria. Food Microbiol. 2001; 18: 261-68.

Cotoros M, Garcia C, Lagos C, Folch C, Mendoza L. Antifungal activity on Botrytis cinerea of flavonoids and diterpenoids isolated from the surface of Pseudognaphalium spp. Bol. Soc. Chil. Quím. 2001; 46.

Duarte MCT, Figueira GM, Sartoratto A, Rehder VLG, Delarmelina C. Anti-candida activity of Brazilian medicinal plants. J Ethnopharmacol. 2005; 97: 305-11.

Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol. 1998; 60: 1-8.

Erturk O. Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants. Biol Bratis. 2006; 61: 275-78.

Fan-Havard P, Capano D, Smith SM, Mangia A, Eng RHK. Development of resistance in Candida isolates from patients receiving prolonged antifungal therapy. Antimicrob Agents Ch. 1991; 35: 2302-05.

Fukai T, Yonekawa M, Hou AJ, Nomura T, Sun HD, Uno J. Antifungal agents from the roots of Cudrania cochinchinensis against Candida, Cryptococcus and Aspergillus species. J Nat Prod. 2003; 66: 1118-20.

Furletti VF, Teixeira IP, Obanda-Perenda G, Mardegan RC, Sartoratto A, Figueira GM, Duarte RMT, Rehder VL, Duarte MCT, Hofling JF. Action of Coriandrum sativum L. essential oil upon oral Candida albicans biofilm formation. Evid Based Complement Altern Med. 2011; 2011: 985832.

Hay RJ. Antifungal therapy and the new azole compounds. J Antimicrob Chemoth. 1991; 28: 36-46.

Hofling JF, Anibal PC, Obanda-Perenda GA, Peixoto IAT, Furletti VF, Foglio MA, Goncalves RB. Antimicrobial potential of some plant extracts against Candida species. Braz J Biol. 2010; 70: 1065-68.

Ioset JR, Marston A, Gupta MP, Hostettmann K. A methylflavan with free radical scavenging properties from Pancratium littorale. Fitoterapia 2001; 72: 35-39.

Kuete V, Mbaveng AT, Tsaffack M, Beng VP, Etoa FX, Nkengfack AE, Marion Meyer JJ, Lall N. Antitumor, antioxidant and antimicrobial activities of Bersama engeleriana (Melianthaceae). J Ethnopharmacol. 2008; 115: 494–501.

Law D, Moore CB, Wardle HM, Ganguli LA, Keaney MGL, Denning DW. High prevalence of antifungal resistance in Candida in AIDS. J Antimicrob Chemoth. 1994; 34: 659–68.

Lewis JR. Amaryllidaceae and Sceletium alkaloids, 1998.

Lin LZ, Hu SF, Chai HB, Pengsuparp T, Pezzuto JM, Cordell GA, Ruangrungsi N. Lycorine alkaloids from Hymenocallis littoralis. Phytochemistry 1995; 40: 1295-98.

Mayachiew P, Devahastin S. Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT-Food Sci Technol. 2008; 41: 1153-59.

Najafi S, Nejad BS. Screening of Pogostemon parviflorus Benth. for anti-candida activity. Afr J Microbiol Res. 2011; 5: 657-60.

National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Susceptibility Testing: Twelfth Informational Supplement M100-S12. NCCLS, Wayne, PA, USA, 2002.

Pettit GR, Pettit III GR, Backhaus RA, Boyd MR, Meerow AW. Antineoplastic agents, 256. Cell growth inhibitory isocarbostyrils from Hymenocallis. J Nat Prod. 1993; 56: 1682-87.

Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM. Phytochemical and antimicrobial activity of Swietenia mahogani crude methanolic seed extract. Trop Biomed. 2009; 26: 274-79.

Tyagi AK, Malik A. In situ SEM, TEM and AFM studies of the antimicrobial activity of lemon grass oil in liquid and vapour phase against Candida albicans. Micron. 2010; 41: 797-805.

Yew CK, Balakrishnan B, Sundarasekar J, Subramaniam S. The effect of cytokinins on in vitro shoot lengths and multiplication of Hymenocallis littoralis. J Med Plants Res. 2010; 4: 2641-46.

Yotsuji A, Shimizu K, Araki H, Fujimaki K, Nishida N, Hori R, Annen Y, Yamamoto S, Hayakawa H, Imaizumi H, Watanbe Y, Narita H. T-8581, a new orally and parenterally active triazole antifungal agent: In vitro and in vivo evaluations. Antimicrob Agents Ch. 1997; 41: 30-34.

Zhang Z, Elsohly HN, Jacob MR, Pasco DS, Walker LA, Clark AM. Natural products inhibiting Candida albicans secreted aspartic proteases from Tovomita krukovi. Planta Med. 2002; 68: 49–54.

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Published

2012-10-03

How to Cite

Sundarasekar, J., G. Sahgal, and S. Subramaniam. “Anti-Candida Activity by Hymenocallis Littoralis Extracts for Opportunistic Oral and Genital Infection Candida Albicans”. Bangladesh Journal of Pharmacology, vol. 7, no. 3, Oct. 2012, pp. 211-6, doi:10.3329/bjp.v7i3.11625.

Issue

Vol. 7 No. 3 (2012)

Section

Research Articles

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