Implication of ermCX gene of Corynebacterium striatum in macrolide resistance in Beijing, China

Authors

  • Wei Li Department of Clinical Laboratory Medicine, People?s Hospital of Peking University, Beijing 100044
  • Zheng Zhang Department of Clinical Laboratory Medicine, People?s Hospital of Peking University, Beijing 100044

DOI:

https://doi.org/10.3329/bjp.v8i1.13169

Keywords:

Antibacterial resistance, Corynebacterium striatum, ermCX, PCR

Abstract

To study the resistance mechanism of Corynebacterium striatum clinical isolates to macrolide antibiotic, 57 strains of C. striatum clinical isolates were detected by susceptibility testing, PCR amplification and DNA sequencing. The MICs of erythromycin and azithromycin were measured. The Staphylococcus aureus (ATCC 29213) strain was used as control strain for susceptibility testing. A novel pair of specific primers for C. striatum was firstly designed. Out of the 57 strains of C. striatum isolates there were 9 strains of ermCX-carrying isolates screened. The amplified ermCX gene of C. striatum was characterized. High MICs (?256 mg/L) of erythromycin and azithromycin was showed in 9 strains which carried ermCX gene. Thus, ermCX gene plays an important role in resistant mechanism of C. striatum in China to macrolide antibiotic. The ermCX gene DNA sequences of two strains (one C. striatum from blood and one C. striatum from pleural fluid) were submitted to GenBank database.

Downloads

Download data is not yet available.
Abstract
207
Download
119 Podcast
39 Read
2

References

Campanile F, Carretto E, Barbarini D, Grigis A, Falcone M, Goglio A, Venditti M, Stefani S. Clonal multidrug-resistant Corynebacterium striatum strains, Italy. Emerg Infect Dis. 2009; 15: 75-78.

Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility testing for uncommon isolates or bacteria that grow aerobically; Approved Standards. CLSI documents M45-A. Clinical and Laboratory Standards Institute, 2010.

de Arriba JJ, Blanch JJ, Mateos F, Martínez-Alfaro E, Solera J. Corynebacterium striatum first reported case of prosthetic valve endocarditis. J Infect. 2002; 44: 193.

Douthwaite S, Jalava J, Jakobsen L. Ketolide resistance in Streptococcus pyogenes correlates with the degree of rRNA dimethylation by Erm. Mol Microbiol. 2005; 58: 613-22.

Douthwaite S, Jensen RL, Kirpekar F. The activity of rRNA resistance methyltransferases assessed by MALDI Mass Spectrometry. Methods Mol Med. 2008; 142: 223-37.

Fernández-Ayala M, Nan DN, Fariñas MC. Vertebral osteomyelitis due to Corynebacterium striatum. Am J Med. 2001 ; 111: 167.

Li W, Zhang Z. The identification and resistance analysis to 66 strains of corynebacterium clinical isolates. Chin J Lab Diag. 2007; 11: 932-36.

Martín MC, Melón O, Celada MM, Alvarez J, Méndez FJ, Vázquez F. Septicaemia due to Corynebacterium striatum: molecular confirmation of entry via the skin. J Med Microbiol. 2003; 52: 599-602.

Otsuka Y, Ohkusu K, Kawamura Y, Baba S, Ezaki T, Kimura S. Emergence of multidrug-resistant Corynebacterium striatum as a nosocomial pathogen in long-term hospitalized patients with underlying diseases. Diagn Microbiol Infect Dis. 2006; 54: 109-14.

Reig M, Galan J, Baquero F, Perez-Diaz JC. Macrolide resistance in Peptostreptococcus spp. mediated by ermTR: Possible source of macrolide-lincosamide-streptogramin B resistance in Streptococcus pyogenes. Antimicrob Agents Chemother. 2001; 45: 630-32.

Soriano F, Fernández-Roblas R, Calvo R, García-Calvo G. In vitro susceptibilities of aerobic and facultative non-spore-forming Gram-positive bacilli to HMR 3647 (RU 66647) and 14 other antimicrobials. Antimicrob Agents Chemother. 1998; 42: 1028-33.

Tarr PE, Stock F, Cooke RH, Fedorko DP, Lucey DR. Multidrug-resistant Corynebacterium striatum pneumoniae in a heart transplant recipient. Transpl Infect Dis. 2003; 5: 53-58.

Tauch A, Kassing F, Kalinowski J, Pühler A. The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid 1995; 34: 119-31.

Downloads

Additional Files

Published

2013-01-14

How to Cite

Li, W., and Z. Zhang. “Implication of ErmCX Gene of Corynebacterium Striatum in Macrolide Resistance in Beijing, China”. Bangladesh Journal of Pharmacology, vol. 8, no. 1, Jan. 2013, pp. 54-57, doi:10.3329/bjp.v8i1.13169.

Issue

Vol. 8 No. 1 (2013)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).