Anti-proliferative and apoptosis-inducing activities of juglone in LS-174T cells

Authors

  • Qiang Li School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Xue-Ling Zhao School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Juan Sun School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Shou-Gang Jiang Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Gong Xianfeng School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080

DOI:

https://doi.org/10.3329/bjp.v8i1.13174

Keywords:

Apoptosis, Caspase, Juglone, LS-174T cell, NAC

Abstract

Anti-proliferative and apoptosis-inducing effects of juglone in LS-174T cells were investigated. In this study, we showed that juglone inhibited the proliferation of LS-174T cells in a time and dose-dependent manner, treatment of juglone resulted in the activation of caspase-9 and caspase-3, decrease of Bcl-2. N-acetylcysteine significantly attenuate LS-174T cell death induced by juglone (p<0.001). In addition, NAC could reverse caspase-3 and caspase-9 activation, increase expression of Bcl-2 protein. Taken together, these findings indicated that juglone-mediated oxidative injury may act as upstream change, trigger ROS release, Bcl-2 modulation, caspase activation, and consequently leading cell apoptosis in LS-174T cells. In conclusion, these findings suggest that juglone may be an effective way for treating human cancers.

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Published

2013-01-26

How to Cite

Li, Q., X.-L. Zhao, J. Sun, S.-G. Jiang, and G. Xianfeng. “Anti-Proliferative and Apoptosis-Inducing Activities of Juglone in LS-174T Cells”. Bangladesh Journal of Pharmacology, vol. 8, no. 1, Jan. 2013, pp. 65-72, doi:10.3329/bjp.v8i1.13174.

Issue

Vol. 8 No. 1 (2013)

Section

Research Articles

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