Effect of spirulina on the levels of zinc, vitamin E and linoleic acid in the palm skin extracts of people with prolonged exposure to arsenic
DOI:
https://doi.org/10.3329/bjp.v8i1.13694Keywords:
Arsenic, Linoleic acid, Keratosis, Palm skin, Spirulina, Vitamin E, ZincAbstract
Spirulina, a dietary supplement, improves the symptoms of arsenical palmer keratosis. To understand its mechanism of action, palm skin extracts of moderate palmer arsenical keratosis (n=10), arsenic exposed controls (n=10) and healthy volunteers (n=10) were collected before and after treatment with spirulina powder 10 g/day orally for 12 weeks. The mean (±SD) amount of zinc in the palm skin of healthy volunteers was 13.1 ± 5.7 ng/cm2, which was not changed significantly in patients (11.3 ± 5.3 ng/cm2). The amount of vitamin E in healthy volunteers was 6.0 ± 0.3 ng/cm2 which was severely reduced in patients (3.5 ± 0.6 ng/cm2). The amount of linoleic acid was lowered in patient (26.7 ± 17.1 ng/cm2) which was statistically significant in comparison to healthy volunteers (p=0.029). After supplementation of spirulina, zinc level in the palm skin of arsenic exposed controls was increased but it was not statistically significant (p=0.068). The vitamin E and linoleic acid levels were not changed significantly in the skin of palm. In conclusion, arsenical keratosis showed significantly low levels of vitamin E and linoleic acid without any significant change in zinc level. After supplementation of spirulina, low levels of these three compounds were not returned towards the normal levels.
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Ahsan H, Perrin M, Rahman A, Parvez F, Stute M, Zheng Y, Milton AH, Brandt-Rauf P, van Geen A, Graziano J. Associations between drinking water and urinary arsenic levels and skin lesions in Bangladesh. JOEM. 2000; 42: 1195-201.
AOAC (Association of Official Analytical Chemists 25. 006). 12th ed. 1975. p 428.
Baker H, Frank O. Determination in serum tocopherol. In: Practical clinical biochemistry. Gowenlock AH (ed.). 6th ed. London, Heinemann Medical Books, 1968, p 902.
Caussy D (ed). A field guide for detection, management and surveillance of arsenicosis cases. New Delhi, WHO, Regional Office for South-East Asia, 2005.
Chowdhury NJA, Mir Misbahuddin M, Rahman MS. Corn extracts lower tissue arsenic level in rat. Bangladesh Med Res Counc Bull. 2009; 35: 21-25.
Chu DH, Haake AR, Holbrook K, et al. The structure and development of the skin. In: Freedberg IM, Eisen AZ, Wolff K, et al., (eds). Fitzpatricks dermatology in general medicine. 6th ed. New York: McGraw-Hill, 2003; 5888.
Dann WJ, Moore T. The absorption spectra of the mixed fatty acids from cod-liver oil. Nutritional Laboratory, University of Cambridge and Medical Research Council. 1933; 1166-69.
Dina AN, Misbahuddin M. Randomized double-blind trial to evaluate the effectiveness of topical administration of propylene glycol in arsenical palmer keratosis. Bangladesh J Pharmacol. 2010; 5: 98-102.
Fariduddin AKM, Misbahuddin M, Manun MIR, Nahar N. Alcohol extract and residue of spirulina in the prevention of accumulation of arsenic in rats. Bangladesh J Physiol Pharmacol. 2001; 17: 15-17.
Fuwa K, Pulido P, Mckay R, Vallee BL. Determination of zinc in biological materials by atomic absorption spectrophotometry. Anal Chem. 1964; 36: 2407.
Hall AF. Arsenical keratosis disappearing with vitamin A therapy. Arch Derm Syph. 1946; 53:154.
Islam AZMM, Misbahuddin M, Sikdar S, Biswas A K, Islam Z, Hadiuzzaman, Khandker S, Mahmud IA, and Ahmad S A. Randomized controlled trial to evaluate the effectiveness of topical use of salicylic acid for treatment of keratosis in arsenicosis patients. In: Misbahuddin M (ed.), Applied research on arsenic in Bangladesh, Dhaka. World Health Organization (Bangladesh), Director General of Health Services, Govt. of Bangladesh. 2007; pp. 92-100.
Kamaluddin M, Misbahuddin M. Zinc supplement reduces tissue arsenic concentration in rats. Bangladesh Med Res Counc Bull. 2006; 32: 87-91.
Karkos P D, Leong S C, Karkos C D, Sivaji N, and Assimakopoulos D A. Spirulina in Clinical Practice: Evidence-Based Human Applications. Evidence-Based Complementary and Alternative Medicine. 20 August, 2011.
Khan MAR, Misbahuddin MS, Khandker S, Ifthaker-Al-Mahmud. Arsenic estimation in foodstuffs of arsenic exposed areas in Bangladesh. In: Applied research on arsenic in Bangladesh. Misbahuddin M (ed). World Health Organization, Dhaka, 2007, pp 31-42.
King JC, Shames DM, Woodhouse LR. Zinc homeostasis in humans. J Nutr. 2000; 130 Suppl: 1360-66.
Misbahuddin M, Islam AZ, Khandker S, Ifthaker- Al- Mahmud, Islam N, Anjumanara. Efficacy of spirulina extract plus zinc in patients of chronic arsenicosis poisoning: a randomized placebo-controlled study. Clin Toxicol (Phila). 2006; 44: 135-41.
Misbahuddin M, Kamaluddin M. Simultaneous administration of zinc and arsenic enhances accumulation in tissues. BMJ (rapid response) 14 November 2002.
Misbahuddin M, Khandker S, Jakariya M. Arsenic contamination of drinking water and foodstuffs. In: Drinking water contaminants in Bangladesh: Focuses on arsenic, fluoride, pesticides, manganese and cyanobacteria. Misbahuddin M, Khandker S (eds). Lambert Academic Publishing, Germany, 2011, pp 12-57.
Misbahuddin M. Consumption of arsenic through cooked rice. Lancet 361:435-36. 2003.
Nasir M, Misbahuddin M, Ali SMK. Selenium intervention in reducing arsenic levels in different tissues. In: Bangladesh Environment 2002, Proceedings of the 2nd International Conference on Bangladesh Environment. Ahmed MF, Tanveer SA, Badruzzaman ABM (eds). ICBEN-2002, Dhaka, Bangladesh, 2002, pp 343-52.
Rajka G. Surface lipid estimation on the back of the hands in atopic dermatitis. Arch Derm Forsch. 1974; 251: 43-48.
Saraymen R, Kilic E, Suleyman Y. Sweat copper zinc, iron, magnesium, and chromium levels in national wrestler. Inonu Universitesi Tip Fakultesi Dergisi. 2004; 11: 7-10.
Stein J, Kulemeier J, Lembcke B and Caspary W F. Simple and rapid method for determination of short-chain fatty acids in biological materials by high-performance liquid chromatography with ultraviolet detection. J Chromatogr. 1992; 576: 53.
Stephens NG, Parsons A, Schofield PM. Randomized controlled trial of vitamin E in patients with coronary disease: Cambridge Heart Antioxidant Study (CHAOS). Lancet 1996; 347: 781-86.
Tabassum NE. Effect of alpha-lipoic acid on the removal of arsenic from arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 27-32.
Thiele JJ, Traber MG, Packer L. Depletion of human stratum corneum vitamin E: An early and sensitive in vivo marker of UV induced photo-oxidation. J Invest Dermatol. 1998; 110: 756-61.
Thiele JJ, Weber SU, Packer L. Sebaceous gland secretion is a major physiologic route of vitamin E delivery to skin. J Invest Dermatol. 1999; 113: 1006-10.
Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more. Free Radical Biol Med. 2007; 43: 4-15.
Umar BU. Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J Pharmacol. 2007; 2: 27-34.
Wu MM, Chiou HY, Wang TW, Hsueh YM, Wang IH, Chen CJ, Lee TC. Association of blood arsenic levels with increased reactive oxidants and decreased antioxidant capacity in a human population of northeastern Taiwan. Environ Health Perspect. 2001; 109: 1011-17.
Yousuf AKM, Misbahuddin M, Rahman MS. Secretion of arsenic, cholesterol, vitamin E, and zinc from the site of arsenical melanosis and leucomelanosis in skin. Clin Toxicol. 2011; 49: 374-78.
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