Hypoglycemic activity and the activation of phosphorylation of a triterpenoid-rich extract from Euryale shell on streptozotocin-induced diabetic mice

Authors

  • Huaibo Yuan College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei
  • Shaohua Meng College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei
  • Zhaobin Gong College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei
  • Deyi Chen College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei
  • Cuan Zhang School of Bio and Food Engineering, Chuzhou University, Chuzhou

DOI:

https://doi.org/10.3329/bjp.v8i3.14718

Keywords:

Euryale shell, Hypoglycemic, Triterpenoid rich extract

Abstract

In the present study, we examined the hypoglycemic properties and the effective mechanisms of a triterpenoid-rich extract from the Euryale shell (ES) in streptozotocin-induced (STZ) diabetic mice. The hydroalcoholic extract of ES (200, 400 and 600 mg/kg/day) was orally administered to STZ-diabetic mice for 4 weeks. We observed that in the liver of diabetic mice, the ES extract caused a sharp reduction in the gene expression of protein tyrosine phosphatase-1B (PTP1B) but induced the gene expression of phosphatidyl-inositol-3-kinase (PI-3K) and protein kinase B (PKB) compared with that of untreated diabetic mice. Additionally, a significant increase in the phosphorylation of the PKB protein was observed (p<0.01). This was corroborated by the inhibition of PTP1B and by the regulation of glucose uptake via PI-3K activation, which together demonstrate that the reduction of PTP1B can modulate key insulin signaling events downstream of the insulin receptor. 

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References

Ali H, Houghton PJ, Soumyanath A. Alpha-amylase inhibitory activity of some Malaysian plants used to treat diabetes: With particular reference to Phyllanthus amarus. J Ethnopharmacol. 2006; 107: 449-55.

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2010; 33: S62-69.

Brahmachari G. Bio-flavonoids with promising antidiabetic potentials: A critical survey. In: Opportunity, challenge and scope of natural products in medicinal chemistry. Tiwari VK, Mishra BB (eds.). Res Signpost. 2011; 2: 187-212.

Cousin SP, Hugl SR, Wrede CE. Free fatty acid induced inhibition of glucose and insulin-1ike growth factor-1 induced deoxyribonucleic acid synthesis in the pancreatic beta-cell line INS-1. Endoerinology 2001; 142: 229-40.

Farese RV, Sajan MP, Standaert ML. Insulin-densitive protein kinases (atypical protein kinase C protein kinase B/Akt): Actions and defects in obesity and type II diabetes. Exp Biol Med. 2005; 9: 593-605.

Grover JK, Vats V, Yadav S. Effect of feeding aqueous extract of Petrocarpus marsupium on glycogen content of tissues and the key enzymes of carbohydrate metabolism. Mol Cell Biochem. 2002; 241: 53-59.

Lee SE, Ju EM, Kim JH. Antioxidant activity of extracts from Euryale ferox seed. Exp Mol Med. 2002; 34: 100-06.

Limaa CR, Vasconcelosa CFB, Costa-Silvab JH. Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2012; 141: 517-25.

Liu M, Wu K, Mao X, Wu Y, Ouyang J. Astragalus polysaccharide improves insulin sensitivity in KKAy mice: Regulation of PKB/GLUT4 signaling in skeletal muscle. J Ethnopharmacol. 2010; 127: 32-37.

Jill E, Joseph L, Harriet, Regina M, Michael. Reduction of protein-tyrosine phosphatase-1B increases insulin signaling in FAO hepatoma cells. Biochem Biophys Res Commun. 2003; 300: 261-67.

Sugimoto K, Irena B, Shoji M. Early changes in insulin receptor signaling and pain sensation in streptozotocin-induced diabetic neuropathy in rats. J Pain. 2008; 9: 237-45.

King H, Aubert RE, Herman WH. Prevalence, numerical estimates, and projections. Diabetes Care. 1998; 21: 1414.

Kim KT, Yoo KM, Lee JW. Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J Ethnopharmacol. 2007; 111: 443-50.

Maiti R, Das UK, Ghosh D. Attenuation of hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats by aqueous extract of seed of Tarmarindus indica. Biol Pharm Bull. 2005; 28: 1172-76.

Nath BK, Chakraborty AK. Studies on amino acid composition of the seeds of Euryale ferox Salisb. Food Sci Technol. 1985; 22: 293-96.

Rebecca J, Lori L, Sandra L. Reduction of protein tyrosine phosphatase 1B increases insulin-dependent signaling in ob/ob mice. Diabetes 2003; 52: 22-25.

Row LC, Ho JC, Chen CM. Cerebrosides and tocopherol trimers from the seeds of Euryale ferox. J Nat Prod. 2007; 70: 1214-17.

Topping D. Cereal complex carbohydrates and their contribution to human health. J Cereal Sci. 2007; 46: 220-29.

Vasconcelos CFB, Maranhão HML, Batista TM, Carneiro EM, Ferreira F, Costa J, Soares LAL, Sá MDC, Souza TP, Wanderley AG. Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. J Ethnopharmacol. 2011; 137: 1533-41.

Verónica Sancho, María V, Nieves González. Effects of glucagon-like peptide-1 and exendins on kinase activity, glucose transport and lipid metabolism in adipocytes from normal and type-2 diabetic rats. J Mol Endocrinol. 2005; 35: 27-38.

Xiao PG, Xing ST, Wang LW. Immunological aspects of Chinese medicinal plants as antiageing drugs. J Ethnopharmacol. 1993; 38: 159-65.

Xu T, Liu Y, Zeng J, Zhang D. Effect of insulin in combination with selenium on blood glucose and PI3K-mediated GLUT4 expression in skeletal muscle of streptozotocin-induced diabetic rats. Eur Rev Med Pharmacol Sci. 2011; 15: 387-93.

Yoshida T, Okuno A, Tanaka J, Takahashi K, Nakashima R, Kanda S, Ogawa J, Hagisawa Y, Fujiwara T. Metformin primarily decreases plasma glucose not by gluconeogenesis suppression but by activating glucose utilisation in a non-obese type 2 diabetes Goto-Kakizaki rats. Eur J Pharmacol. 2009; 623: 141-47.

Yuan H, Gong Z, Meng S. Hypoglycemic and hypolipidemic effects of a triterpenoid-rich extract from Euryale shell on streptozotocin-induced diabetic mice. Pharmazie. 2013; 68: 227-31.

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Published

2013-05-13

How to Cite

Yuan, H., S. Meng, Z. Gong, D. Chen, and C. Zhang. “Hypoglycemic Activity and the Activation of Phosphorylation of a Triterpenoid-Rich Extract from Euryale Shell on Streptozotocin-Induced Diabetic Mice”. Bangladesh Journal of Pharmacology, vol. 8, no. 3, May 2013, pp. 230-5, doi:10.3329/bjp.v8i3.14718.

Issue

Vol. 8 No. 3 (2013)

Section

Research Articles

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