Dracorhodin perchlorate induces apoptosis in bladder cancer cells through Bcl-2, Bcl-XL, survivin down-regulation and caspase-3 activation

Authors

  • Wei Wei Dental Hospital, Jilin University, Changchun
  • Jun Di Pathology, Jilin Province Hospital, Changchun,
  • Azhar Rasul The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Chaoyue Zhao Dental Hospital, Jilin University, Changchun
  • Faya Martin Millimouno The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Ichiro Tsuji Department of Public Health, Tohoku University, Sendai
  • Furhan Iqal Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan
  • Mahadev Malhi The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Xiaomeng Li The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Jiang Li Dental Hospital, Jilin University, Changchun

DOI:

https://doi.org/10.3329/bjp.v8i3.15025

Keywords:

Apoptosis, Bcl-2, Caspase-3, Dracorhodin Perchlorate, T24 cells

Abstract

Urinary bladder cancer is one of the most commonly diagnosed urological malignancies worldwide. Dracorhodin perchlorate, anthocyanin red pigment, has been recently shown to induce apoptotic cell death in several types of cancer cells. However, there is no report elucidating its effect on bladder cancer T24 cells. In this study, for the first time, we investigated the effects of dracorhodin perchlorate on the cell viability and apoptosis in human bladder cancer T24 cells. DNA flow cytometric analysis demonstrated that dracorhodin perchlorate markedly rendered apoptosis of T24 cells in a time-dependent manner. Dracorhodin perchlorate significantly induced the dissipation of mitochondrial membrane potential in T24 cells. Furthermore, dracor-hodin perchlorate-induced apoptosis was regulated by activation of caspase-3 and down-regulation of antiapoptotic proteins, Bcl-2, Bcl-XL, and survivin in T24 cells. These in vitro results suggested that dracorhodin perchlorate should be further examined for in vivo activity and molecular mechanism in human bladder cancer.

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Author Biography

Azhar Rasul, The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun

Postdoctoral Research Fellow

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Published

2013-06-14

How to Cite

Wei, W., J. Di, A. Rasul, C. Zhao, F. M. Millimouno, I. Tsuji, F. Iqal, M. Malhi, X. Li, and J. Li. “Dracorhodin Perchlorate Induces Apoptosis in Bladder Cancer Cells through Bcl-2, Bcl-XL, Survivin down-Regulation and Caspase-3 Activation”. Bangladesh Journal of Pharmacology, vol. 8, no. 3, June 2013, pp. 276-82, doi:10.3329/bjp.v8i3.15025.

Issue

Vol. 8 No. 3 (2013)

Section

Research Articles

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