Inotropic and chronotropic effects of methanol extract of Aquilaria agallocha on rabbit heart

Authors

  • Nawazish-i-Husain Syed University College of Pharmacy, University of the Punjab, Lahore
  • Qaisar Mahmood Department of Pharmacy, University of Sargodha, Sargodha
  • Maqsood Ahmad Department of Pharmacy, Bahauddin Zakariya University, Multan
  • Muhammad Shoib Akhtar Department of Pharmacy, University of Sargodha, Sargodha

DOI:

https://doi.org/10.3329/bjp.v8i3.15080

Keywords:

β1-adrenoceptors, Coronary flow, Force of contraction, Heart rate, Langendorff, Voltage-gated Ca2 channel

Abstract

Aquilaria agallocha Roxb. (Thymelaeceae) heartwood is used in cardiac failure; however, its cardiotonic properties are poorly understood at a pharmacological level. This study investigated methanol extract of A. agallochafor presence of glycosides and its effects on force of contraction, heart rate and coronary flow on rabbit isolated heart. Furthermore, contribution of β1-adrenoceptors and/or L-type Ca2+ channels in A. agallocha-induced cardiac effects was explored. A. agallocha extract, digoxin, verapamil and metoprolol were administered in a retrograde manner. Force of contraction and heart rate were recorded with a force transducer attached to the heart. Coronary flow was measured from collected effluent. The extract induced a significant increase in force of contraction (p<0.001), decrease in heart rate (p<0.05) and coronary flow (p<0.001). Metoprolol (p<0.01) and verapamil (p<0.01) significantly inhibited the plant extract-induced effects. Thus, A. agallocha exhibited cardiotonic effects, most likely via cardiac glycosides, involving ?1-adrenoceptors and L-type Ca2+ channels.

Downloads

Download data is not yet available.
Abstract
414
Download
184 Read
3

Author Biographies

Nawazish-i-Husain Syed, University College of Pharmacy, University of the Punjab, Lahore

Assistant Professor

Qaisar Mahmood, Department of Pharmacy, University of Sargodha, Sargodha

Lecturer

Maqsood Ahmad, Department of Pharmacy, Bahauddin Zakariya University, Multan

Professor

Muhammad Shoib Akhtar, Department of Pharmacy, University of Sargodha, Sargodha

Professor

References

Ahmad M, Syed NH, Akhtar MS, Erum A, Mahmood Q, Alamgeer. Iono- and chronotropic effects of aqueous extract of Berberis lyceum Royale root bark and berberine on in stiu frog-heart preparation. Lat Am J Pharm. 2012; 31: 756-60.

Boskabady MH, Shafei MN, Shakiba A, Sefidi HS. Effect of aqueous-ethanol extract from Crocus sativus (saffron) on guinea-pig isolated heart. Phytother Res. 2008; 22: 330-34.

Consolini AE, Ragone MI, Migliori GN, Conforti P, Volonte MG. Cardiotonic and sedative effects of Cecropia pachystachya Mart. (ambay) on isolated rat hearts and conscious mice. J Ethnopharmacol. 2006; 106: 90-96.

Dash M, Patra JK, Panda PP. Phytochemical and antimicrobial screening of extracts of Aquilaria agallocha Roxb. Afr J Biotech. 2008; 7: 3531-34.

Dias C, Graca JAB, Goncalves ML. Scilla maderensis, TLC screening and positive inotropic effect of bulb extracts. J Ethnopharmacol. 2000; 71: 487-92.

Garjani A, Afrooziyan A, Nazemiyeh H, Najafi M, Kharazmkia A, Maleki-Dizaji N. Protective effects of hydroalcoholic extract from rhizomes of Cynodon dactylon (L.) Pers. on compensated right heart failure in rats. BMC Complement Altern Med. 2009; 9: 28.

Gilani AH, Janbaz KH, Aziz N, Herzig MJU, Kazmi MM, Choudhary MI, Herzig JW. Possible mechanism of selective inotropic activity of the n-butanolic fraction from Berberis aristata fruit. Gen Pharmacol. 1999; 33: 407-14.

Gunasekera SP, Kinghorn AD, Cordell GA, Farnsworth NR. Plant anticancer agents, XIX. Constituents of Aquilaria malaccensis. J Nat Prod. 1981; 44, 569-72.

Jermsri P, Jiraviriyakul A, Unajak S, Kumphune S. Effects of Aquilaria crassna crude extract on stimulated ischemia induced cardiac cell death. Int J Pharm Bio Sci. 2012; 3: 604-13.

Jermsri P, Kumphune S. Ethylacetate extract of Aquilaria crassna preserve actin cytoskeleton on stimulated ischemia induced cardiac cell death. J Med Plants Res. 6: 2012; 4057-62.

Kim YC, Lee EH, Lee YM, Kim HK, Song BK, Lee EJ, Kim HM. Effect of the aqueous extract of Aquilaria agallocha stems on the immediate hypersensitivity reactions. J Ethnopharmacol. 1997; 58: 31-38.

Kitada Y, Narimatsu A, Suzuki R, Endoh M, Taira N. Does the positive inotropic action of a novel cardiotonic agent, MCI-154, involve mechanisms other than cyclic AMP? J Pharmacol Exp Ther. 1987; 243: 639-45.

Kumar AR, Subburathinam KM, Prabakar G. Phytochemical screening of selected medicinal plants asclepiadaceae family. Asian J Microbial Biotechnol Enviorn Sci. 2007; 9: 177-80.

Kumphune S, Jermsri P. An in vitro anti-ischemic effect of Aquilaria crassna in isolated rat ventricular myocytes subjected to stimulated ischemia. J Phytother Pharmacol. 2012; 1: 47-54.

Lokhande PD, Dhaware BS, Jagdale SC, Chabukswar AR, Atre AL. Chronotropic and inotropic effects of Piper longum Linn. Trends Appl Sci Res. 2006; 1, 634-39.

Miniyar BP, Chitre TS, Karve SS, Deuskar HJ, Jain KS. Antioxidant activity of ethylacetate extract of Aquilaria agellocha on nitrite-induced methemoglobin formation. Int J Green Pharm. 2008; 1: 43-44.

Mohire NC, Salunkhe VR, Bhise SB, Yadav AV. Cardiotonic activity of aqueous extract of heartwood of Pterocarpus marsupium. Indian J Exp Biol. 45: 532-37.

Okugawa, H, Ueda R, Matsumoto K, Kawanishi K, Katao A. Effects of agarwood extracts on the central nervous systems in mice. Planta Med. 1993; 59: 32-36.

Patrick LO, Timothy J. Antioxidants in medicines and spices as cardioprotective agents in Tibetan highlanders. Pharm Biol. 2002; 40: 346-57.

Pennacchio M, Syah YM, Ghisalberti EL, Alexander E. Cardioactive compounds from Eremophila species. J Ethnopharmacol. 1996; 53: 21-27.

Riebliny PW, Walker GC. Extractions and extractives, in: Remingtons Pharmaceutical Sciences. Lippincott Williams & Wilkins, Maryland, USA, 2006.

Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan Nigeria, Spectrum Books, 1982, p 6, 154.

Woo AYH, Waye MMY, Kwan HS, Chan MC, Chau CF, Cheng CHK. Inhibition of ATPases by Cleistocalyx operculatus: A possible mechanism for the cardiotonic actions of the herb. Vasc Pharmacol. 2002; 38: 163-68.

Zhou M, Wang H, Suolangjiba, Kou J, Yu B. Antinociceptive and anti-inflammatory activities of Aquilaria sinensis (Lour.) Gilg. leaves extract. J Ethnopharmacol. 2008; 117: 345-50.

Downloads

Additional Files

Published

2013-07-09

How to Cite

Syed, N.- i-H., Q. Mahmood, M. Ahmad, and M. S. Akhtar. “Inotropic and Chronotropic Effects of Methanol Extract of Aquilaria Agallocha on Rabbit Heart”. Bangladesh Journal of Pharmacology, vol. 8, no. 3, July 2013, pp. 311-6, doi:10.3329/bjp.v8i3.15080.

Issue

Vol. 8 No. 3 (2013)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).