First report of anticancer agent, lapachol producing endophyte, Aspergillus niger of Tabebuia argentea and its in vitro cytotoxicity assays
DOI:
https://doi.org/10.3329/bjp.v9i1.15622Keywords:
Antimitotic, Antiproliferative, Aspergillus niger, Tabebuia argenteaAbstract
All parts of Tabebuia argentia were used for isolation and identified the lapachol producing endophytes were used for evaluation of in vitro cytotoxicity (antimitotic, antiproliferative, determination of cell viability, DNA fragmentation). Five endophytes (leaf endophytes, Alternaria alternata, Alternaria sp., Aspegerillus niger, Penicillium sp. and the bark endophyte, A. alternata) are able to produce potent anticancer agent lapachol. The 3rd and 4th fractions of endophytic extracts (A. niger and Penicillium sp.) exhibited the pure lapachol. The 3rd fraction of A. niger lapachol strongly inhibited the Allium cepa root actively growing cells at various stages of cell cycle in antimitotic assay and the index was 22.5 mg/mL, the yeast cells were died due to toxicity and possessed the cell necrosis and they exhibited the DNA fragmentation. Present promised endophytes can be used for production of lapachol using endophyte A. niger for large scale production of lapachol within short period of time.
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References
Almeida ER, Filha AAS, Santos ERD, Lopes CAC. Anti-inflammatory action of lapachol. J Ethnopharmacol. 1990; 29: 239-41.
Baker D, Mocek U, Garr C. Natural products vs. combinatorials: A case study. In: Biodiversity: New leads for pharmaceutical and agrochemical industries. Wrigley SK, Hayes MA, Thomas R, Chrystal EJT, Nicholson N (ed). United Kingdom, The Royal Society of Chemistry, 2000, pp 66-72.
Balassiano II, Paulo SA, Silva NH, Cabral MC, Carvalho MC. Demonstration of lapachol as a potential drug for fighting cancer metastases. Oncol Rep. 2005; 13: 329.
Barnett HL, Hunter BB. Illustrated genera of imperfect fungi. II ed. Burgess Publishing Company. Minnesota. 1972.
Bicas JL, Neri-Numa IA, Ruiz ALTG, De Carvalho JE, Pastore GM. Evaluation of the antioxidant and antiproliferative potential of bioflavors. Food Chem Toxicol. 2011; 49: 1610-15.
Bhujbal SS, Deshmukh RP, Bidkar JS, Thatte VA, Awasare SS, Garg PP. Evaluation of cytotoxicity activity of barks of Mimosops elengi. EurAsian J BioSci. 2011; 5: 73-79.
Breger J, Fuchs BB, Aperis G, Moy TI, Ausubel FM, Mylonakis E. Antifungal chemical compounds identified using a C. elegans pathogenicity assay. Plos Pathog. 2007; 3: 168-78.
Bussaban B, Lumyong S, Lumyong P, McKenzie EH, Hyde KD. Endophytic fungi from Amomum siamense. Canadian J Microbiol. 2001; 47: 943-48.
Da Silva MN, Da Souza MCBV, Ferreira VF, Pinto AV, Pinto MCRF, Solange MSV, Wardell SMSV, Wardell JL. Synthesis of new aldehyde derivatives from β-lapachone and nor-β-lapachone. Arkivoc. 2003; 156-68.
Houghton PJ, Photiou A, Uddin S, Shah P, Browning M, Jackson SJ, Retsas S. Activity of extracts of Kigelia pinnata against melanoma and renal carcinoma cell lines. Planta Med. 1994; 60: 430-33
Jangala M, Manche S, Mudigonda S, Raja MK, Sangras BR, Konagurtu V. Evaluation of cytotoxicity of atenolol in Allium cepa L. Int J Toxicol Appl Pharmacol. 2012; 2: 18-24.
Lee JC, Strobel GA, Lobkovsky E, Clardy JC. Torreyanic acid: A selectively cytotoxic quinone dimer from the endophytic fungus Pestalotiopsis microspora. J Org Chem. 1996; 61: 3232-33.
Li JY, Stroble GA, Sidhu R, Hess WM, Ford EJ. Endophytic Taxol producing fungi from bald cypress, Taxodium distichum. Microbiology. 1996; 142: 2223-26.
Murray MT, Pizzorno JE. Encyclopedia of natural medicine 2nd ed. PA4 Rocklin, CA. Prima Pub 1998; pp. 967-72.
Ping KY, Darah I, Yusuf UK, Sasidharan S. Genotoxicity of Euphorbia hirta on Allium cepa assay. Int Conf Nutr Food Sci. 2012; 39: 222-25.
Raviraja NS, Maria GL, Sridhar KR. Antimicrobial evaluation of endophytic fungi inhabiting medicinal plants of the Western Ghats of India. English Life Sci. 2006; 6515-20.
Sadananda TS, Nirupama R, Chaithra K, Govindappa M, Chandrappa CP, Vinay Raghavendra B. Antimicrobial and antioxidant activities of endophytes from Tabebuia argentea and identification of anticancer agent (lapachol). J Med Plant Res. 2011; 5: 3643-52.
Sehgal R, Roy S, Kumar VL. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allum cepa root model. J Biocell. 2006; 30: 9-13.
Shweta S, Khadabadi S, Ganesh TG. In vitro antimitotic, antiproliferative, DNA fragmentation and anticancer activity of chloroform and ethanol extract of Revia hypocrarerformis. Asian Pacific J Trop Dis. 2012; S503-06.
Silva TMS, Camara CA, Barbosa TP, Soares AZ, Cunha LC, Pinto AC, Vargas MD. Design, synthesis and antifungal activity of a novel water soluble prodrug of antifungal triazole. Bioorganic Med Chem Lett. 2005; 13: 193-96.
Stierle A, Strobel GA, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 1993; 260: 214-16.
Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev.2003; 67: 491-502
Subhadradevi V, Khairunissa K, Asokkumar K, Umamaheswari M, Sivashanmugam A, Jagannath P. Induction of apoptosis and cytotoxic activities of Apium graveolens Linn. using in vitro models. Middle-East J Sci Res. 2011; 9: 90-94.
Teixeira MJ, Almeida YM, Viana JR, Holanda Filha JG, Rodrigues TP, Prata JRC, Jr, Coelho ICB, Rao VS, Pompeu MML. In vitro and in vivo leishmanicidal activity of 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol). Phytother Res. 2001; 15: 44-48.
Theantana T, Hyde KD, Lumyong S. Aspaginase production by endophytic fungi from Thai medicinal plants: Cytotoxic properties. Int J Integra Biol. 2009; 7: 1-8.
Thomson RH. Naturally Occurring Quinones, III. Recent advances. London, Chapman and Hall, 1987, 142, pp 144-47.
Türk M, Kaya B, Menemen Y, Oğuztüzün S. Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro.J Med Plants Res. 2011; 5: 4566-71.
Visalakchi S, Muthumary J. Taxol (anticancer drug) producing endophytic fungi: An overview. Int J Pharma Bio Sci. 2010; 1: 1-9.
Wuerzberger SM, Pink JJ, Planch SM, Byers KL, Bornmann WG, Boothman DA. Induction of apoptosis in MCF-7:WS8 breast cancer cells by /3-lapachone1. Cancer Res. 1998; 58: 1876-85.
Yu SF, Chen TM, Chen YH. Apoptosis and necrosis are involved in the toxicity of Sauropus androgynus in an in vitro study. J Formos Med Assoc. 2007; 106: 537-47.
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