The colony count of Escherichia coli in the stool of palmar arsenical keratosis following probiotics supplementation

Authors

  • Nafisa Rashid Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • Mir Misbahuddin Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • Zubaida Khatoon Choudhry Marks Medical College, Mirpur, Dhaka
  • Ahmed Abu Saleh Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • Abu Naser Ibne Sattar Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

DOI:

https://doi.org/10.3329/bjp.v9i2.17168

Keywords:

Arsenic, E. coli, Keratosis, Probiotic

Abstract

The role of probiotics supplementation for 12 weeks on the colony count of Escherichia coli in the stool of moderate palmar arsenical keratosis (14), arsenic exposed controls (15) and healthy volunteers (9) were studied. The mean colony counts of E. coli in the stool of arsenic exposed controls and patients were reduced to 7.3 x 1011 CFU/g and 3.2 x 1011 CFU/g respectively in comparison to healthy volunteers (11.1 x 1011 CFU/g). After probiotics supplementation, the E. coli count was significantly increased in patients (p=0.000) and arsenic exposed controls (p=0.000) in comparison to healthy volunteers (p=0.027). Patients excreted less arsenic through stool and supplementation with probiotics showed increased excretion of arsenic only in patients  The level of arsenic in nail of patients was more than the arsenic exposed controls and healthy controls. Probiotics supplementation did not show any significant change. This study suggests that arsenicosis reduced the stool colony count of E. coli, less excretion of arsenic and probiotics supplementation reversed the processes.

Downloads

Download data is not yet available.
Abstract
289
Download
136 Read
1

References

Ahmad SA, Faruquee MH, Sayed MHSU, Khan MH, Hadi SA, Khan AW. Chronic arsenic poisoning management by vitamin A, E, C regimen. J Pre Social Med (JOPSOM). 1998; 17: 19-26.

AOAC (Association of Official Analytical Chemists 25. 006) 12th ed. 1975, p 428.

Bashar T, Misbahuddin M, Hossain MA. A double-blind, randomize, placebo-control trial to evaluate the effect of Nigella sativa on palmer arsenical keratosis patients. Bangladesh J Pharmacol. 2014; 9: 15-21.

Bentley R, Chasteen TG. Microbial methylation of metalloids: Arsenic, antimony, and bismuth. Microbiol Mol Biol Rev. 2002; 66: 250-71.

Boyle RJ, Browne RMR and Tang MLK. Probiotic use in clinical practice: What are the risks? Am J Clin Nutr. 2006; 83: 1256-64.

Buchanan K, Heimbach DM, Minshew BH, Coyle MB. Comparison of quantitative and semi-quantitative culture techniques for burn biopsy. J Clin Microbiol. 1986; 23: 258-61.

Chowdhury NJA, Mir Misbahuddin M, Rahman MS. Corn extracts lower tissue arsenic level in rat. Bangladesh Med Res Counc Bull. 2009; 35: 21-25.

Choudhury ZK, Misbahuddin M, Hosain AKMM, Saleh AA. Inhibitory effect of arsenic on aerobic gut flora in rat. Bangladesh Med Res Counc Bull. 2009; 35: 79-83.

Chowdhury UK, Biswas BK, Chowdhury TR, Samanta G, Mandal BK, Basu GC, Chanda CR, Lodh D, Saha KC, Mukherjee SK, Roy S, Kabir S, Quamruzzaman Q, Chakraborti D. Groundwater Arsenic Contamination in Bangladesh and West Bengal, India. Environ Health Perspect. 2000; 108: 393-97.

Gamble MV, Liu X, Ahsan H, Pilsner JR, Ilievski V, Slavkovich V, Parvez F, Levy D, Litvak PF, Graziano JH. Folate, homocysteine and arsenic metabolism in arsenic exposed individuals in Bangladesh. Environ Health Perspect. 2005; 113: 1683-88.

Hall AF. Arsenical keratosis disappearing with vitamin A therapy. Arch Derm Syph. 1946; 53: 154.

Kamaluddin M, Misbahuddin M. Zinc supplement reduces tissue arsenic concentration in rats. Bangladesh Med Res Counc Bull. 2006; 32: 87-91.

Misbahuddin M, Khandker S, Jakariya M. Arsenic contamination of drinking water and foodstuffs. In: Drinking water contaminants in Bangladesh: Focuses on arsenic, fluoride, pesticides, manganese and cyanobacteria. Misbahuddin M, Khandker S (eds). Germany, Lambert Academic Publishing, 2011, pp 12-57.

Misbahuddin M, Bashar T, Hossain MA. Effectiveness of garlic oil in the treatment of arsenical palmer keratosis. Bangladesh J Pharmacol. 2013; 8: 22-27.

Nasir M, Misbahuddin M, Ali SMK. Selenium intervention in reducing arsenic levels in different tissues. In: Bangladesh Environment 2002, Proceedings of the 2nd International Conference on Bangladesh Environment. Ahmed MF, Tanveer SA, Badruzzaman ABM (eds). ICBEN-2002, Dhaka, Bangladesh, 2002, pp 343-52.

Qin J, Rosen BP, Zhang Y, Wang G, Frankie S, Rensing C. Arsenic detoxification and evolution of trimethyle arsine gas by a microbial arsenite S-adenosylmethionine methyltransferase. Proc Natl Acad Sci. 2006; 103: 2075-80.

Rahman MF, Misbahuddin M. Effect of folic acid and tetrahydrofolate on tissue arsenic level in rat. Bangladesh J Pharmacol. 2010; 5: 25-29.

Rahman MM, Chowdhury UK, Mukherjee SC, Mondal BK, Paul K, Lodh D, Biswas K, Chanda CR, Basu GK, Saha KC, Roy S, Das R, Palit SK, Quamruzzaman Q, Chakraborti D. Chronic arsenic toxicity in Bangladesh and West Bengal, India: A review and commentary. J Toxicol Clin Toxicol. 2001; 39: 683-700.

Rowland IR, Davies MJ. In vitro metabolism of inorganic arsenic by the gastrointestinal microflora of the rat. J Appl Toxicol. 1981; 1: 278-83.

Saha B. Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 68-71.

Salminen S, Bouley C, Rault MCB, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau MC, Roberfroid M, Rowland I. Functional food science and gastrointestinal physiology and function. Br J Nutr. 1998; 80: 147-71.

Stylbo M, Drobna Z, Jasper I, Lin S, Thomas DJ. The role of biomethylation in toxicity and carcinogenecity of arsenic: A research update. Environ Health Perspect. 2002; 110: 767-71.

Tabassum NE. Effect of alpha-lipoic acid on the removal of arsenic from arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 27-32.

Umar BU. Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J Pharmacol. 2007; 2: 27-34.

Upreti RK, Kannan A, Shivastava R, Chaturvedi UC. A comparative study on rat intestinal epithelial cells and resident gut bacteria (ii) effect of arsenite. Biol Med Environ Sci. 2006; 19: 77-86.

Upreti RK, Shivastava R, Chaturvedi UC. Gut microflora and toxic metals: Chromium as a model. Indian Med Res. 2004; 119: 49-59.

Vahter M. Mechanisms of arsenic biotransformation. Toxicology 2002; 181: 211-17.

Downloads

Additional Files

Published

2014-04-09

How to Cite

Rashid, N., M. Misbahuddin, Z. K. Choudhry, A. A. Saleh, and A. N. I. Sattar. “The Colony Count of Escherichia Coli in the Stool of Palmar Arsenical Keratosis Following Probiotics Supplementation”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, Apr. 2014, pp. 176-81, doi:10.3329/bjp.v9i2.17168.

Issue

Vol. 9 No. 2 (2014)

Section

Clinical Trial

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).