Hepatoprotective activity of aqueous methanolic extract of Viola odorata against paracetamol-induced liver injury in mice
DOI:
https://doi.org/10.3329/bjp.v9i2.18049Keywords:
Hepatoprotective, Jaundice, Silymarin, Viola odorataAbstract
Traditionally Viola odorata is used for liver protection. To provide scientific support to its traditional use, aqueous methanolic extract of V. odorata (250 mg/kg and 500 mg/kg) was given to mice intoxicated with paracetamol. Obtained results demonstrated that the extract significantly (p<0.01-0.001) reduced paracetamol induced increase levels of serum hepatic enzymes and total bilirubin. Histopathological studies showed that the plant attenuated the hepatocellular necrosis and inflammation. HPLC results showed the presence of hepatoprotective flavonoids (isorhamnetin and luteolin) in the extract. It was concluded from the present study that V. odorata has hepatoprotective activity against paracetamol-induced liver injury in mice.
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Ahmad M, Mahmood Q, Gulzar K, Akhtar MS, Saleem M, Qadir MI. Antihyperlipidaemic and hepatoprotective activity of Dodonaea viscosa leaves extracts in alloxan-induced diabetic rabbits (Oryctolagus cuniculus). Pakistan Vet J. 2012; 32: 50-54.
Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of antioxidant and hepatoprotective effects of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8: 44-48.
Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, Ahmed B. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2013; 8: 300-04.
Amin N, Qadir MI, Khan TJ, Abbas G, Ahmad B, Janbaz KH, Ali M. Antibacterial activity of Vacuum liquid chromatography (VLC) isolated fractions of chloroform extracts of seeds of Achyranthes aspera. J Chem Soc Pak. 2012; 34: 589-92.
Amiri MS, Joharchi MR, Taghavizadeh Yazdi ME. Ethnomedicinal plants used to cure jaundice by traditional healers of Mashhad, Iran. Iranian J Pharm Res. 2014; (Ahead of print)
Arora DS, Kaur GJ. Antibacterial activity of some Indian medicinal plants. Natl Med J. 2007; 61: 313-17.
Asif MA, Qadir MI. Molecular approaches for development of malarial vaccines. Rev Pharmacol. 2011; 4: 276-78.
Bag AK, Mumtaz SMF. Hepatoprotective and nephroprotective activity of hydroalcoholic extract OfIpomoea staphylina leaves. Bangladesh J Pharmacol. 2013; 8: 263-68.
Balamurugan G, Muthusamy P. Observation of the hepatopro-tective and anti-oxidant activities of Trianthema decandra Linn. (Vallai sharunnai) roots on carbon tetrachloride-treated rats. Bangladesh J Pharmacol. 2008; 3: 83-89.
Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract. J Ethnopharmacol. 2003; 89: 217-19.
Ehsan O, Qadir MI, Malik SA, Abbassi WS, Ahmad B. Efficacy of nanogold-insulin as a hypoglycemic agent. J Chem Soc Pakistan. 2012; 34: 365-70.
Hegde K, Joshi AB. Hepatoprotective and antioxidant effect of Carissa spinarum root extract against CCl4 and paracetamol-induced hepatic damage in rats. Bangladesh J Pharmacol. 2010; 5: 73-76.
Hussain A, Khalid SH, Qadir MI, Massud A, Ali M, Khan IU, Saleem M, Iqbal MS, Asghar S, Gul H. Water uptake and drug release behaviour of methyl methacrylate-coitaconic acid [P(MMA/IA)] hydrogels cross-linked with methylene bis-acrylamide. J Drug Deliv Sci Tech. 2011; 21: 249-55.
Janbaz KH, Nisar U, Ashraf M, Qadir MI. Spasmolytic, bronchodilator and anti-oxidant activities of Erythrina superosa Roxb. Acta Pol Pharm. 2012; 69: 1111-17.
Janbaz KH, Saeed SA, Gilani, AH. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine 2004; 11: 424-30.
Javed F, Qadir MI, Janbaz KH, Ali M. Novel drugs from marine microorganisms. Cr Rev Microbiol. 2011; 37: 245-49.
Khalid SH, Qadir MI, Massud A, Ali M, Rasool MH. Effect of degree of cross-linking on swelling and drug release behaviour of poly (methyl methacrylate-coitaconic acid) [P(MMA/IA)] hydrogels for site specific drug delivery. J Drug Deliv Sci Tech. 2009; 19: 413-18.
Khan AZ, Mohammad A, Iqbal Z, Anis I, Shah MR, Nadeem S, Rabnawaz M, Shahidullah A, Khan H, Khan I. Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from Dodonaea viscose. Bangladesh J Pharmacol. 2013; 8: 36-39.
Khattak SG, Gilani SN, Ikram M. Antipyretic studies on some indigenous Pakistani medicinal plants. J Ethnopharmacol. 1985; 14: 45-51.
Koochek M, Pipelzadeh M, Mardani H. The effectiveness of Viola odorata in the prevention and treatment of formalin induced lung damage in the rat. J Herbs Spices Med Plants. 2003; 10: 95-103.
Masood MI, Qadir MI, Shirazi JH, Khan IU. Beneficial effects of lactic acid bacteria on human beings. Cr Rev Microbiol. 2011; 37: 91-98.
Naz S, Qadir MI, Ali M, Janbaz KH. Nanotechnology for imaging and drug delivery in cancer. J Chem Soc Pakistan. 2011; 34: 107-11.
Qadir MI, Malik SA. Anti-diabetic activity of inorganic metals Eugenia jambolana Lam. (Myrtaceae) flowers. Pharmacologyonline 2010; 2: 979-85.
Qadir MI, Malik SA. Effect of Eugenia jambolana leaves extracts on blood glucose levels of experimental diabetic rabbits. Pharmacologyonline. 2009; 3: 829-35.
Qadir MI. Medicinal and cosmetological importance of Aloe vera. Int J Nat Ther. 2009; 2: 21-26.
Qadir MI. Medicinal values of ginger. Int J Nat Ther. 2009; 3: 19-22.
Qadir MI. Qadirvirtide. Pakistan J Pharm Sci. 2011; 24: 593-95.
Sabir SM, Rocha JBT. Anti-oxidant and hepatoprotective activity of aqueous extract of Solanum fastigiatum against paracetamol-induced liver damage in mice. J Ethno-pharmacol. 2008; 120: 226-32.
Saboo SS, Tapadiya G, Farooqui IA, Khadabadi SS. Free radical scavenging, in vivo anti-oxidant and hepatoprotective activity of folk medicine Trichodesma sedgwickianum. Bangladesh J Pharmacol. 2013; 8: 58-64.
Saddiqe Z, Naeem I, Maimoona A, Sautreau A, Hellio C. Assay of flavonoid aglycones with HPLC in four species of genus Hypericum. J Med Plants Res. 2011; 5: 1526-30.
Sadeque MZ, Begum ZA. Protective effect of dried fruits of Carica papaya on hepatotoxicity in rat. Bangladesh J Pharmacol. 2010; 5: 48-50.
Saleem TM, Chetty CM, Ramkanth S, Rajan VST, Kumar KM and Gauthaman K. Hepatoprotective herbs: A review. Int J Res Pharm Sci. 2010; 1: 1-5.
Siddiqi HS, Mehmood MH, Rehman NU, Gilani AH. Studies on the antihypertensive and antidyslipidemic activities of Viola odorata leaves extract. Lipids Health Dis. 2012; 11: 6.
Singab ANB, Youssef DT, Noaman E, Kotb S. Hepatoprotective effect of flavonol glycosides rich fraction from Egyptian Vicia calcarata desf. Against CCI4-induced liver damage in rats. Arch Pharmacal Res. 2005; 28: 791-98.
Thakare SP, Jain HN, Patil SD, Upadhyay UM. Hepatoprotective effect of Cocculus hirsutus on bile duct ligation-induced liver fibrosis in albino Wistar rats. Bangladesh J Pharmacol. 2009; 4: 126-30.
Vadivu R, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchnensis (Lour) Moore ssp laurina. Bangladesh J Pharmacol. 2008; 3: 121-24.
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