Cardioprotective effect of Trichopus zeylanicus against myocardial ischemia induced by isoproterenol in rats
DOI:
https://doi.org/10.3329/bjp.v4i2.1824Keywords:
Isoproterenol, Lipid peroxidation, Myocardial ischemia, Trichopus zeylanicusAbstract
The cardioprotective effects of Trichopus zeylanicus leaves against isoproterenol-induced myocardial ischemia was studied. Wister strain rats were pretreated with T. zeylanicus leaves (500 mg/kg body weight) for 28 days and then intoxicated with isoproterenol (20 mg/100 g, i.p. for 2 consecutive days). Cardioprotection was assessed by estimating plasma and heart aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and creatine phosphokinase. Troponin T was estimated in serum, the levels of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) were analyzed in heart and plasma. In isoproterenol-treated group, shrinkage of cardiac markers in plasma and elevated lipid peroxidation where accompanied by decreased content of reduced glutathione in heart and plasma. The prior administration of T. zeylanicus significantly (p < 0.001) prevented the isoproterenol-induced alterations and restored the cardiac markers. These findings indicate the cardioprotective activities of T. zeylanicus during isoproterenol-induced myocardial ischemia.
Downloads
159
182 Read
2
References
Beuge JA, Aust SD. The thiobarbituric acid assays. Method Enzymol. 1978; 52: 302-10.
Bhaskar I, Rao SB. New, simple and cheap alternative to troponin test for diagnosis of acute myocardial infarction. Indian J Exp Biol. 2002; 40: 628-30.
Evans DA, Subramoniam A, Rajasekharan S, Pushpangadan P. Effect of Trichopus zeylanicus Gaertn, leaf extract on the energy metabolism in mice during exercise and at rest. Indian J Pharmacol. 2002; 34: 3237.
Handforth CP. Isoproterenol-induced myocardial infarction in animals. Arch Pathol. 1962; 73: 161-65.
Harvey J, Paige SM. The instat guide to choosing and interpreting statistical tests: A manual for graph pad instat. Version 3. San Diego, CA USA. 1998.
King J. The dehydrogenases or oxidoreductase: Lactate dehydrogenase. In: Practical clinical enzymology. Nostrand V (ed). London. 1965, p 106.
Kurian GA, Philp S, Varghese T. Effect of aqueous extract of Desmodium gangeticum DC root in the severity of myocardial infarction. J Ethanopharmcol. 2005; 97: 4557-61.
Lakshmi B, Tilak JC, Adhikari S, Devasagayam TPS, Janardhanan KK. Inhibition of lipid peroxidation induced by Gamma-radiation and AAPH in rat liver and brain mitiochondria by mushrooms. Curr Sci. 2005; 88: 484-88.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folins reagent. J Biol Chem. 1951; 193: 265-76.
Moron MS, DsePierre JW, Manerwik KB. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochim Biophy Acta. 1979; 582: 67-68.
Nigam PK. Biochemical markers of myocardial injury. Indian J Clin Biochem. 2007; 22: 10-17.
Okinaka S, Kumaggai H, Ebashi S, Sugita H, Momoi H, Toyokura Y, Fujie Y. Serum creatine phosphokinase. Activity in progressive muscular dystrophy and neuromuscular diseases. Arch Neurol. 1961; 4: 520-25.
Prabhu S, Jainu M, Sabitha KE, Shyamala Devi CS. A beneficial effect of Maniferin on isoproterenol-induced myocardial infarction in rat model. Phcog Mag. 2005; 1: 92-96.
Pushpangadan P, Rajasekharan S, Rathesh Kumar PK, Jawahar CRVNV, Lakshmi N, Amma, LS. Arogyappacha, (Trichopus zeylanicus Gaertn) The ginseng of Kani tribes of Agashtyar Hills (Kerala) for Ever Green. 1988.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamate oxaloacetic and glutamate pyruvate transaminases. Am J Clin Pathol. 1957; 28: 56-63.
Senthil S, Sridevi M, Pugalendi KV. Protective effect of ursolic acid against myocardial ischemia induced by isoproterenol in rats. Toxicol Mechm Methd. 2007; 17: 57-65.
Sharma AK, Pushpangandan P, Chopra CL, Rajasekharan S. Adaptogenic activity of seeds of Trichopus zeylanicus Gaertn. The ginseng of Kerala. Anc Sci Life. 1989; 8: 212-19.
Singh B, Chandan BK, Sharma N, Singh S, Khajuria A, Gupta DK. Adaptogenic activity of glyco-peptido-lipid fraction from the alcoholic extract of Trichopus zeylanicus Gaerten (part II). Phytomedicine 2005; 12: 468-81.
Singh B, Gupta, DK, Chandan BK. Adaptogenic activity of a glycopeptido-lipid fraction from the alcoholic extract of Trichopus zeylanicus Gaertn. Phytomedicine 2001; 8: 283-91.
Subramoniam A, Madhavachandra V, Rajasekharan S, Pushphangadan P. Apradosiac property of Trichopus zeylanicus in male mice. J Ethanopharmcol. 1997; 57: 21-27.
Additional Files
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).