Hepatoprotective activity of Thymus linearis against paracetamol and carbon tetrachloride-induced hepatotoxicity in albino mice
DOI:
https://doi.org/10.3329/bjp.v9i2.18329Keywords:
Anti-oxidant, Hepatoprotective, Thymus linearisAbstract
In the present study, the hepatoprotective activity of aqueous and ether extracts of Thymus linearis (250 and 500 mg/kg orally) was evaluated against carbon tetrachloride- and paracetamol-induced hepatic damage in mice. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) were assessed. Antioxidant activity of both the extracts was also determined using 1-1-diphenyl-2-picryl hydrazine (DPPH) scavenging method. The results indicated that both the extracts significantly produce a dose dependent reduction in serum levels of ALT, AST, ALP when compared to carbon tetrachloride-and paracetamol-treated groups. The maximum effect in all the parameters was observed at a dose of 500 mg/kg. The extracts also demonstrated a significant antioxidant activity. LD50 of both extracts was found to be a 1050 and 900 mg/kg respectively. It is conceivable that the hepatoprotective activity of T. linearis might be due to the presence of certain pharmacologically active compounds.
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Aghel N, Kalantari H, Rezazadeh S. Hepatoprotective effect of Ficus carica leaf extract on mice intoxicated with carbon tetrachloride. Irani J Pharm Sci. 2011; 10: 63-68.
Ahsan R, Islam KM, Musaddik A, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbontetrachloride induced toxicity in albino rats. Global J Pharmacol. 2009; 3: 116-22.
Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of anti-oxidant and hepatoprotective effects of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8: 44-48.
Akhtar MS, Nadeem M, Rashid HU, Bashir S. Hypoglycemic activity of different fractions of Berberis aristata root-bark in normal and alloxan diabetic rabbits. Can J of App Sci. 2011; 1: 16-28.
Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, Ahmad B. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2013; 8: 300-04.
Bag AK, Mumtaz SMF. Hepatoprotective and nephroprotective activity of hydroalcoholic extract of Ipomoea staphylina leaves. Bangladesh J Pharmacol. 2013; 8: 263-68.
Balamurugan G, Muthusamy P. Observation of the hepatoprotective and anti-oxidant activities of Trianthema decandra Linn. (Vallaisharunnai) roots on carbon tetrachloride-treated rats. Bangladesh J Pharmacol. 2008; 3: 83-89.
Guntupalli M. Hepatoprotective effects of rubiadin, a major constituent of Rubia Cordifolia. J Ethnopharmacol. 2006; 103: 484-90.
Hegde K, Joshi AB. Hepatoprotective and anti-oxidant effect of Carissa spinarumroot extract against CCl4 and paracetamol-induced hepatic damage in rats. Bangladesh J Pharmacol. 2010; 5: 73-76.
Ivorra S, Paya M, Villar A. A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol. 1989; 27: 243-75.
Kanchana N, Sadiq AM. Hepatoprotective effect of Plumbago zeylanica on paracetamol-induced liver toxicity in rats. Int J Pharm Pham Sci. 2011; 3: 151-54.
Khandelwal KR. Practical pharmacognosy. 12th ed. Pune, Nirali Prakashan Publishers, 2006, 149-53.
Khan AZ, Mohammad A, Iqbal Z, Anis I, Shah MR, Nadeem S, Rabnawaz M, Shahidullah A, Khan H, Khan I. Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from Dodonaea viscose. Bangladesh J Pharmacol. 2013; 8: 36-39.
Kshirsagar AD, Mohite R, Aggrawal AS, Suralkar UR. Hepatoprotective medicinal plants of Ayurveda: A review. Asian J Pharmaceut Clin Res. 2011; 4: 1-8.
Maheswari C, Maryammal R, Venkatanarayanan S. Hepato-protective activity of Orhosiphon stamineus on liver damage caused by paracetamol in rats. JJBS. 2008; 1: 105-08.
Mittal DK, Joshi D, Shukla S. Protective effects of Polygonum bistorta (Linn.) and its active principle against acetaminophen-induced toxicity in rats. Asian J Exp Biol Sci. 2010; 1: 951-58.
Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA. Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr. 2007; 40: 163-73.
Molyneux PH. The use of stable free radical diphenyl picrylhydrazyl (DPPH) for estimating anti-oxidant activity. Songklanakarin J Sci Tech. 2004; 26: 211-19.
Nadeem MPC, Dandiya PC, Pasha M, Imran D, Balani K, Vohora SB. Hepatoprotective activity of Solanum nigrum fruits. Fitoterapia 1997; 68: 245-51.
NRC. Guide for the care and use of laboratory animals. Washington DC, National Academy Press, 1996.
Periasamy M, Pavankumar K, Gangadhar V, Jeeva T, Anandhan R, Sengottuvelu S. Hepatoprotective and anti-oxidant activity of Euphorbia ligularia against carbon tetrachloride induced hepatotoxicity in Wistar rats. Int J Res Pharm Biomed Sci. 2012; 3: 100-04.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Anti-oxidant activity, phenol and flavonoids contents of some selected Iranian medicinal plants. Afr J Biotech. 2006; 5: 42-1145.
Qureshi LR. Ethnobotanical studies of selected medicinal plants of Sudhan Gali and Ganga Chotti Hills, District, Azad Kashmir. Pak J Bot. 2007; 39: 2275-83.
Rajesh MG, Latha MS. Hepatoprotection by Elephatopus scaber Linn in CCl4 induced liver injury. Indian J Physiol Pharmacol. 2001; 45: 481-86.
Rehman JU, Saqib NU, Akhtar N, Jamshaid M, Asif HM, Sultana S, Rehman RU. Hepatoprotective activity of aqueous-methanolic extract of Suaeda fruticosa in paracetamol-induced hepatotoxicity in rabbits. Bangladesh J Pharmacol. 2013; 8: 378-81.
Saboo SS, Tapadiya G, Farooqui IA, Khadabadi SS. Free radical scavenging, in vivo antioxidant and hepatoprotective activity of folk medicine Trichodesma sedgwickianum. Bangladesh J Pharmacol. 2013; 8: 58-64.
Sharma PK, Lal B. Ethnobotanical notes on some medicinal and aromatic plants of Himachal Pradesh. Indian J Tradit Know. 2005; 4: 424-28.
Shetty A, Shyamjith, Deepa, Alwar, MC. Acute toxicity studies and determination of median lethal dose. Current Sci. 2007; 93: 917-20.
Wakchaure D, Jain D, Singhai AK, SomanI R. Hepatoprotective activity of Symplocos racemosa Roxb. bark extract in carbon tetrachloride induced liver damage in rats. J Ayurveda Integr Med. 2011; 2: 137-43.
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