Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Limonia elephantum leaves
DOI:
https://doi.org/10.3329/bjp.v9i3.19704Keywords:
Limonia elephantum, Antioxidant, Hepatoprotective, AcetaminophenAbstract
Limonia elephantum (wood apple) has been widely used in an Indian folk medicine system. In the present study, the acute toxicity studies were carried out to determine the safety of the compounds in liver diseases. The antioxidant and the hepatoprotective properties of the L. elephantum are evaluated against paracetamol induced hepatic damage in rats. Liver superoxide dismutase, lipid peroxidation, glutathione peroxidase, catalase levels and serum biochemical profile such as serum glutamate oxalate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, bilirubin and total protein were examined for the antioxidant and hepatoprotective effects of these treatments. The results of the present studies strongly indicate that the higher dose (400 mg/kg) of ethanolic extract of L. elephantum proved against hepatoprotective effects and also the antioxidant properties.
Downloads
337
156 Read
504
References
Aebi H. Catalase. In: Methods in enzymatic analysis. Bergmeyar HU (eds). New York, Academic Press, 1974, pp 674-84.
Alamgeer, Nawaz M, Ahmad T, Mushtaq MN, Batool A. Hepatoprotective activity of Thymus linearis against paracetamol and carbon tetrachloride-induced hepatotoxicity in albino mice. Bangladesh J Pharmacol. 2014; 9: 230-34.
Chance B, Green Stein DS, Roughton RJW. The mechanism of catalase action I-steady state analysis. Arch Biochem Biophys. 1952; 37: 301- 39.
Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys. 1959; 82: 70-77.
Erdelmeier I, Gerard-Monnier D, Yadan JC, Acudiere J. Reactions of N-methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals: Mechanistic aspects of the colorimetric assay of lipid peroxidation. Chem. Res. Toxicol.1998; 11: 118494.
Ghosh MN, Kale SR. Evaluation of analgesic activity. In: Fundamentals of experimental pharmacology. 2nd ed, Scientific book Agency, Calcutta, 2005, pp 69-71.
Gornall AG, Bardwill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949; 177: 751-56.
Hiroshini A, Toshiharu H, Masahiro H, Sholi. An alteration in liver microsomal membrane of the rat following paracetamol overdose. J Pharm Pharmacol. 1987; 39: 1047-49.
Hussain L, Akash MSH, Tahir M, Rehman K, Ahmed KZ. Hepatoprotective effects of methanolic extract of Alcea rosea against acetaminophen-induced hepatotoxicity in mice. Bangladesh J Pharmacol. 2014; 9: 322-27.
Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984; 21: 131-32.
Kamalakannan K, Balakrishnan V. Ethanobotanical studies on Achyranthes Aspera Linn. Among the folk peoples of Tamil Nadu, South India. J Phytol. 2009; 1: 108-11.
King EJ, Armstrong AR. A convenient method for determining of serum and bile phosphatase activity. J Canad. Med. Assoc. 1934; 31: 376-81.
Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem. Biophys. Res. Commun. 1976; 71: 95258.
Mallhi TH, Abbas K, Ali M, Qadir MI, Saleem M, Khan YH. Hepatoprotective activity of methanolic extract of Malva parviflora against paracetamol-induced hepatotoxicity in mice. Bangladesh J Pharmacol. 2014; 9: 342-46.
Malloy HT, Evelyn KA. The determination of bilirubin with the photometric colorimeter. J Biol Chem. 1937; 119: 481-90.
Moss DW, Butterworth PJ. Enzymology and Medicine. Pitman Medical, London, 1974, pp 139-42.
Ohkawa H, Onishi N, Yagi K. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. J Anal Biochem. 1979; 95: 351-58.
Prakash J, Gupta SK, Kochupillai V, Singh N, Gupta YK, Joshi S. Chemopreventive activity of Withania somnifera in experimentally induced fibrosarcoma tumours in swiss albino mice. Phytother Res. 2001; 15: 240-44.
Qadir MI, Murad MSA, Ali M, Saleem M, Farooqi AA. Hepatoprotective effect of leaves of aqueous ethanol extract of Cestrum nocturnum against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2014a; 9: 167-70.
Qadir MI, Ali M, Ali M, Saleem M, Hanif M. Hepatoprotective activity of aqueous methanolic extract of Viola odorata against paracetamol-induced liver injury in mice. Bangladesh J Pharmacol. 2014b; 9: 198-202.
Retimen S, Frankel SA. Colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvate trans-aminases. Am J Clin Pathol. 1957; 28: 56-63.
Saleem M, Ahmed B, Qadir MI, Mahrukh, Rafiq M, Ahmad M, Ahmad B. Hepatoprotective effect of Chenopodium murale in mice. Bangladesh J Pharmacol. 2014a; 9: 124-28.
Saleem M, Ahmed B, Karim M, Ahmed S, Ahmad M, Qadir MI, Syed NIH. Hepatoprotective effect of aqueous methanolic extract of Rumex dentatus in paracetamol-induced hepatotoxicity in mice. Bangladesh J Pharmacol. 2014b; 9: 284-89.
Scott MD, Lubin BH, Zuo L, Kuypers FA. Erythrocyte defense against hydrogen peroxide: Preeminent importance of catalase. J Lab Clin Med. 1991; 118: 7-16.
Veereshwarayya V, Thiruvengadam D. Hepatoprotective effect of allicin on tissue defense system in galactosamine/ endotoxin challenged rats. J Ethnopharmacol. 2004; 90: 151-54.
Wagh SS, Shinde GB. Antioxidant and hepatoprotective activity of Tridax Procumbens Linn, against paracetamol-induced hepatotoxicity in male albino rats. Adv Stu Biol. 2010; 2: 105-12.
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
