Structural characterizations of lead anticancer compounds from the methanolic extract of Jatropha tanjorensis

Authors

  • Purushothaman K. Arun Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu
  • Sivarathri S. Rajesh Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu
  • Sankaranarayanan M. Sundaram Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu
  • Thirunavukkarasu Sivaraman Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu
  • Pemiah Brindha Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu

DOI:

https://doi.org/10.3329/bjp.v9i4.19771

Keywords:

Anti-cancer, Apoptosis, In silico, Jatropha tanjorensis, Vitexin

Abstract

LC/ESI/MS/MS data analysis on the phytoconstituents of methanolic extract of Jatropha tanjorensis leaves revealed the presence of abundant flavone glucosides (homoorientin, kaempferol-3-o-rutinoside, luteolin-7-o-glucoside, 6-C-pentosyl-8-C-hexosyl apigenin, naringin and vitexin), flavonol (kaempferol and kaempferide) and flavones (baicalein and diosmetin). We, herein, demonstrated that methanolic extract of J. tanjorensis possess DPPH free-radical scavenging activity (IC50 of 49.7 µg/mL), inhibition of  lipid peroxidation activity (IC50 of 189.6 µg/mL) and anticancer activity through MTT assay against EAC cells (IC50 of 14.57 µg/mL) and Caco-2 cells (IC50 of 21.0 µg/mL). In silico analysis indicated that cytotoxic activity of the methanolic extract of J. tanjorensis could be attributed to the presence of vitexin and 6-C-pentosyl-8-C-hexosyl apigenin as evidenced by exhaustive molecular docking studies carried out against 8 proteins of Bcl-2 family that play essential role in apoptosis. Moreover, drug-likeness properties of the leads and scopes to develop them as potent anticancer compounds are discussed.

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Author Biographies

Purushothaman K. Arun, Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu

Research Scholar

Sivarathri S. Rajesh, Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu

Research Scholar

Sankaranarayanan M. Sundaram, Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu

Student

Thirunavukkarasu Sivaraman, Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu

Associate Professor

Pemiah Brindha, Centre for Advanced Research in Indian Systems of Medicine, SASTRA University, Thanjavur 613401, Tamil Nadu

Associate Dean and Coordinator

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Additional Files

Published

2014-09-30

How to Cite

Arun, P. K., S. S. Rajesh, S. M. Sundaram, T. Sivaraman, and P. Brindha. “Structural Characterizations of Lead Anticancer Compounds from the Methanolic Extract of Jatropha Tanjorensis”. Bangladesh Journal of Pharmacology, vol. 9, no. 4, Sept. 2014, pp. 452-65, doi:10.3329/bjp.v9i4.19771.

Issue

Vol. 9 No. 4 (2014)

Section

Research Articles

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